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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2008-3-19
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pubmed:abstractText |
Positively charged residues located near the cytoplasmic end of hydrophobic segments in membrane proteins promote membrane insertion and formation of transmembrane alpha-helices. A quantitative understanding of this effect has been lacking, however. Here, using an in vitro transcription-translation system to study the insertion of model hydrophobic segments into dog pancreatic rough microsomes, we show that a single Lys or Arg residue typically contributes approximately -0.5 kcal/mol to the apparent free energy of membrane insertion (DeltaG(app)) when placed near the cytoplasmic end of a hydrophobic segment and that stretches of 3-6 Lys residues can contribute significantly to DeltaG(app) from a distance of up to approximately 13 residues away.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-10590301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-11356190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-11700072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-14661030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-14668483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-15028726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-15136048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-15461443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-15663952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-15674282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-15681341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-15723347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-16291756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-16453726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-16928357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-17008929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-17531810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-17893139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-18046402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-18075582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-2677744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-3560218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-5592400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-8510652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-8611495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/18326626-9490057
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1091-6490
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
18
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pubmed:volume |
105
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4127-32
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pubmed:dateRevised |
2010-9-22
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pubmed:meshHeading |
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pubmed:year |
2008
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pubmed:articleTitle |
Contribution of positively charged flanking residues to the insertion of transmembrane helices into the endoplasmic reticulum.
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pubmed:affiliation |
Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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