Source:http://linkedlifedata.com/resource/pubmed/id/17934699
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2008-1-24
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| pubmed:abstractText |
Oxidative stress is detrimental to sperm function and a significant factor in the etiology of male infertility. Present study evaluates the effect of ter butyl hydroperoxide (TBHP)-induced oxidative stress on the spermatogenic process and cell number in the seminiferous tubules. Intraperitoneal injection of TBHP (84 mumol TBHP/100 g body weight) for 2 weeks to male Balb/c mice resulted in enhanced lipid peroxidation (P < 0.0001) decrease in reduced glutathione (P < 0.0001) and increase in the oxidized glutathione levels (P = 0.007) in the testis. Status of spermatogenesis after the treatment was assessed by the quantitative methods of germ cell evaluation in the seminiferous tubules. A significant decrease in the number of young spermatids (P = 0.0003) and pachytene cells (P = 0.022) was observed. A marked reduction was also seen in the mature spermatid number (P < 0.0001). An increase in testicular mRNA levels of redox-regulated cjun (P = 0.008) and cfos (P = 0.0006) subunits of activator protein 1 (AP1) was observed after TBHP treatment. Evaluation of AP1 regulated antioxidant enzymes in the testis revealed an increase in gamma-glutamyl cysteine synthetase (GCS) mRNA expression (P = 0.001). These results suggest a potential role of AP1 in oxidative stress-mediated meiotic and post meiotic changes in the spermatogenic process and regulation of cell number in male reproductive system.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/tert-Butylhydroperoxide
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0300-8177
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
308
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
177-81
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| pubmed:meshHeading |
pubmed-meshheading:17934699-Animals,
pubmed-meshheading:17934699-Cell Count,
pubmed-meshheading:17934699-Glutathione,
pubmed-meshheading:17934699-Kinetics,
pubmed-meshheading:17934699-Lipid Peroxidation,
pubmed-meshheading:17934699-Male,
pubmed-meshheading:17934699-Mice,
pubmed-meshheading:17934699-Oxidation-Reduction,
pubmed-meshheading:17934699-Oxidative Stress,
pubmed-meshheading:17934699-RNA, Messenger,
pubmed-meshheading:17934699-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17934699-Sertoli Cells,
pubmed-meshheading:17934699-Spermatogenesis,
pubmed-meshheading:17934699-Testis,
pubmed-meshheading:17934699-Transcription Factor AP-1,
pubmed-meshheading:17934699-Transcription Factors,
pubmed-meshheading:17934699-Up-Regulation,
pubmed-meshheading:17934699-tert-Butylhydroperoxide
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| pubmed:year |
2008
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| pubmed:articleTitle |
Upregulation of AP1 by tertiary butyl hydroperoxide induced oxidative stress and subsequent effect on spermatogenesis in mice testis.
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| pubmed:affiliation |
Department of Biophysics, Panjab University, Chandigarh 160014, India.
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| pubmed:publicationType |
Journal Article
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