Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-7-27
pubmed:abstractText
Epitope mapping of a MHC class I-restricted cytotoxic T cell response to nef, a regulatory protein of HIV, was performed with fresh PBMC from HIV-seropositive donors and target cells pulsed with a panel of overlapping peptides of the nef protein. These nef-specific CTL recognized a synthetic peptide of 10 residues derived from a nonamphipathic, highly conserved region of the nef protein in association with the HLA A3.1 molecule. Using human cell transfectants expressing mutations of the A3 molecule, we demonstrated that the amino acid at position 152 of the A3.1 molecule appears to be critical for detection of this response. Thus, rapid analysis of the epitopes of HIV proteins stimulating CTL responses can be achieved using a combination of fresh donor PBMC and target cells pulsed with synthesized peptides.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
145
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
127-35
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:1694201-Amino Acid Sequence, pubmed-meshheading:1694201-Cytotoxicity, Immunologic, pubmed-meshheading:1694201-DNA Mutational Analysis, pubmed-meshheading:1694201-Epitopes, pubmed-meshheading:1694201-Gene Products, nef, pubmed-meshheading:1694201-HIV Antibodies, pubmed-meshheading:1694201-HIV Antigens, pubmed-meshheading:1694201-HIV-1, pubmed-meshheading:1694201-HLA-A3 Antigen, pubmed-meshheading:1694201-Humans, pubmed-meshheading:1694201-Immunity, Cellular, pubmed-meshheading:1694201-Major Histocompatibility Complex, pubmed-meshheading:1694201-Molecular Sequence Data, pubmed-meshheading:1694201-Recombinant Proteins, pubmed-meshheading:1694201-T-Lymphocytes, Cytotoxic, pubmed-meshheading:1694201-Transfection, pubmed-meshheading:1694201-Viral Regulatory and Accessory Proteins, pubmed-meshheading:1694201-nef Gene Products, Human Immunodeficiency Virus
pubmed:year
1990
pubmed:articleTitle
Mapping the fine specificity of a cytolytic T cell response to HIV-1 nef protein.
pubmed:affiliation
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
pubmed:publicationType
Journal Article