| pubmed-article:16339486 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16339486 | lifeskim:mentions | umls-concept:C0032105 | lld:lifeskim |
| pubmed-article:16339486 | lifeskim:mentions | umls-concept:C1306889 | lld:lifeskim |
| pubmed-article:16339486 | lifeskim:mentions | umls-concept:C0134835 | lld:lifeskim |
| pubmed-article:16339486 | lifeskim:mentions | umls-concept:C1749467 | lld:lifeskim |
| pubmed-article:16339486 | lifeskim:mentions | umls-concept:C1154393 | lld:lifeskim |
| pubmed-article:16339486 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:16339486 | lifeskim:mentions | umls-concept:C0442818 | lld:lifeskim |
| pubmed-article:16339486 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:16339486 | pubmed:dateCreated | 2006-1-6 | lld:pubmed |
| pubmed-article:16339486 | pubmed:abstractText | Raised levels of soluble P-selectin (sP-selectin) have been reported in the plasma of patients with vascular diseases; however, the functional importance of this ligand remains unclear. In this study we have examined a potential role for plasma sP-selectin in regulating neutrophil adhesion in patients with peripheral arterial occlusive disease (PAOD). Patients with PAOD had significantly higher levels of sP-selectin (mean+/-SD: 73.3+/-13.0 versus 16.7+/-6.4 ng/mL) and enhanced whole blood leukocyte adhesion to platelets under shear. To examine whether the raised sP-selectin levels can directly influence leukocyte adhesion, isolated neutrophils were incubated with plasma from PAOD patients before and after immunodepletion of sP-selectin. Neutrophil adhesion to fibrinogen increased 2-fold following incubation with PAOD plasma, which was abrogated on sP-selectin immunodepletion. We subsequently demonstrated that recombinant sP-selectin dose-dependently (75 to 250 ng/mL) increased leukocyte adhesion to fibrinogen and platelet monolayers. This increase was PSGL-1 and Src kinase-dependent and correlated with an increase in sP-selectin-mediated Mac-1 activation. sP-selectin-stimulated neutrophil adhesion to platelet monolayers was inversely correlated with shear, such that at low shear (50 s(-1)) a 92.7%+/-15.7 increase in adhesion was observed decreasing to 38.5%+/-11.9 at 150 s(-1) and 10.1%+/-7.4 at 300 s(-1). These studies suggest a potentially important role for sP-selectin in modulating neutrophil adhesion in patients with PAOD, particularly at sites of low shear, where it raises the possibility that raised plasma sP-selectin levels may enhance leukocyte recruitment to vascular injury and promote disease progression. | lld:pubmed |
| pubmed-article:16339486 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16339486 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16339486 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16339486 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16339486 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16339486 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16339486 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16339486 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:16339486 | pubmed:issn | 1524-4571 | lld:pubmed |
| pubmed-article:16339486 | pubmed:author | pubmed-author:KulkarniSS | lld:pubmed |
| pubmed-article:16339486 | pubmed:author | pubmed-author:KlingDD | lld:pubmed |
| pubmed-article:16339486 | pubmed:author | pubmed-author:JacksonSS | lld:pubmed |
| pubmed-article:16339486 | pubmed:author | pubmed-author:DartA MAM | lld:pubmed |
| pubmed-article:16339486 | pubmed:author | pubmed-author:Chin-DustingJ... | lld:pubmed |
| pubmed-article:16339486 | pubmed:author | pubmed-author:WoollardK JKJ | lld:pubmed |
| pubmed-article:16339486 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:16339486 | pubmed:day | 6 | lld:pubmed |
| pubmed-article:16339486 | pubmed:volume | 98 | lld:pubmed |
| pubmed-article:16339486 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16339486 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16339486 | pubmed:pagination | 149-56 | lld:pubmed |
| pubmed-article:16339486 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16339486 | pubmed:meshHeading | pubmed-meshheading:16339486... | lld:pubmed |
| pubmed-article:16339486 | pubmed:meshHeading | pubmed-meshheading:16339486... | lld:pubmed |
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| pubmed-article:16339486 | pubmed:meshHeading | pubmed-meshheading:16339486... | lld:pubmed |
| pubmed-article:16339486 | pubmed:meshHeading | pubmed-meshheading:16339486... | lld:pubmed |
| pubmed-article:16339486 | pubmed:meshHeading | pubmed-meshheading:16339486... | lld:pubmed |
| pubmed-article:16339486 | pubmed:meshHeading | pubmed-meshheading:16339486... | lld:pubmed |
| pubmed-article:16339486 | pubmed:meshHeading | pubmed-meshheading:16339486... | lld:pubmed |
| pubmed-article:16339486 | pubmed:meshHeading | pubmed-meshheading:16339486... | lld:pubmed |
| pubmed-article:16339486 | pubmed:year | 2006 | lld:pubmed |
| pubmed-article:16339486 | pubmed:articleTitle | Raised plasma soluble P-selectin in peripheral arterial occlusive disease enhances leukocyte adhesion. | lld:pubmed |
| pubmed-article:16339486 | pubmed:affiliation | Alfred and Baker Medical Unit, Wynn Domain, Baker Heart Research Institute, Melbourne, Australia. Kevin.Woollard@baker.edu.au | lld:pubmed |
| pubmed-article:16339486 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16339486 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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