| pubmed-article:16301669 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16301669 | lifeskim:mentions | umls-concept:C0521026 | lld:lifeskim |
| pubmed-article:16301669 | lifeskim:mentions | umls-concept:C0027950 | lld:lifeskim |
| pubmed-article:16301669 | lifeskim:mentions | umls-concept:C1711178 | lld:lifeskim |
| pubmed-article:16301669 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:16301669 | lifeskim:mentions | umls-concept:C1155266 | lld:lifeskim |
| pubmed-article:16301669 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:16301669 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:16301669 | pubmed:dateCreated | 2005-11-22 | lld:pubmed |
| pubmed-article:16301669 | pubmed:abstractText | Previously, we elucidated the intracellular mechanisms by which neutrophil elastase (NE) up-regulates inflammatory gene expression in bronchial epithelial cells. In this study, we examine the effects of both IL-1 and NE on inflammatory gene expression in 16HBE14o- bronchial epithelial cells and investigate approaches to abrogate these inflammatory responses. IL-1 induced IL-8 protein production in time- and dose-dependent fashions, an important observation given that IL-8 is a potent neutrophil chemoattractant and a key inflammatory mediator. IL-1 and NE were shown to activate the p38 MAPK pathway in 16HBE14o- cells. Western blot analysis demonstrated IL-1R-associated kinase 1 (IRAK-1) degradation in response to stimulation with both IL-1 and NE. In addition, the expression of dominant negative IRAK-1 (IRAK-1delta), IRAK-2delta, or IRAK-4delta inhibited IL-1- and NE-induced NF-kappaB-linked reporter gene expression. Dominant negative versions of the intracellular adaptor proteins MyD88 (MyD88delta) and MyD88 adaptor-like (Mal P/H) abrogated NE-induced NF-kappaB reporter gene expression. In contrast, only MyD88delta was found to inhibit IL-1-induced NF-kappaB reporter activity. We also investigated the vaccinia virus proteins, A46R and A52R, which have been shown to antagonize IL-1 signaling. Transfection with A46R or A52R cDNA inhibited IL-1- and NE-induced NF-kappaB and IL-8R gene expression and IL-8 protein production in primary and transformed bronchial epithelial cells. Furthermore, cytokine array studies demonstrated that IL-1 and NE can up-regulate the expression of IL-6, oncostatin M, epithelial cell-derived neutrophil activating peptide-78, growth-related oncogene family members, vascular endothelial growth factor, and GM-CSF, with induction of these proteins inhibited by the viral proteins. These findings identify vaccinia virus proteins as possible therapeutic agents for the manifestations of several inflammatory lung diseases. | lld:pubmed |
| pubmed-article:16301669 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16301669 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16301669 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16301669 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:16301669 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:16301669 | pubmed:author | pubmed-author:TaggartCliffo... | lld:pubmed |
| pubmed-article:16301669 | pubmed:author | pubmed-author:GreeneCatheri... | lld:pubmed |
| pubmed-article:16301669 | pubmed:author | pubmed-author:McElvaneyNoel... | lld:pubmed |
| pubmed-article:16301669 | pubmed:author | pubmed-author:O'NeillShane... | lld:pubmed |
| pubmed-article:16301669 | pubmed:author | pubmed-author:CarrollTomás... | lld:pubmed |
| pubmed-article:16301669 | pubmed:author | pubmed-author:BowieAndrew... | lld:pubmed |
| pubmed-article:16301669 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16301669 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:16301669 | pubmed:volume | 175 | lld:pubmed |
| pubmed-article:16301669 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16301669 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16301669 | pubmed:pagination | 7594-601 | lld:pubmed |
| pubmed-article:16301669 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:meshHeading | pubmed-meshheading:16301669... | lld:pubmed |
| pubmed-article:16301669 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16301669 | pubmed:articleTitle | Viral inhibition of IL-1- and neutrophil elastase-induced inflammatory responses in bronchial epithelial cells. | lld:pubmed |
| pubmed-article:16301669 | pubmed:affiliation | Respiratory Research Division, Royal College of Surgeons in Ireland, Education and Research Center, Beaumont Hospital, Dublin, Ireland. | lld:pubmed |
| pubmed-article:16301669 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16301669 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
