{
  "http://linkedlifedata.com/resource/pubmed/id/15882069" : {
    "http://linkedlifedata.com/resource/pubmed/abstractText" : [ {
      "value" : "S100B is a dimeric Ca(2+)-binding protein that undergoes a 90 +/- 3 degrees rotation of helix 3 in the typical EF-hand domain (EF2) upon the addition of calcium. The large reorientation of this helix is a prerequisite for the interaction between each subunit of S100B and target proteins such as the tumor suppressor protein, p53. In this study, Tb(3+) was used as a probe to examine how binding of a 22-residue peptide derived from the C-terminal regulatory domain of p53 affects the rate of Ca(2+) ion dissociation. In competition studies with Tb(3+), the dissociation rates of Ca(2+) (k(off)) from the EF2 domains of S100B in the absence and presence of the p53 peptide was determined to be 60 and 7 s(-)(1), respectively. These data are consistent with a previously reported result, which showed that that target peptide binding to S100B enhances its calcium-binding affinity [Rustandi et al. (1998) Biochemistry 37, 1951-1960]. The corresponding Ca(2+) association rate constants for S100B, k(on), for the EF2 domains in the absence and presence of the p53 peptide are 1.1 x 10(6) and 3.5 x 10(5) M(-)(1) s(-)(1), respectively. These two association rate constants are significantly below the diffusion control ( approximately 10(9) M(-)(1) s(-)(1)) and likely involve both Ca(2+) ion association and a Ca(2+)-dependent structural rearrangement, which is slightly different when the target peptide is present. EF-hand calcium-binding mutants of S100B were engineered at the -Z position (EF-hand 1, E31A; EF-hand 2, E72A; both EF-hands, E31A + E72A) and examined to further understand how specific residues contribute to calcium binding in S100B in the absence and presence of the p53 peptide.",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/grant" : [ {
      "value" : "http://linkedlifedata.com/resource/pubmed/grant/GM58888",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/grant/F30-NS043916",
      "type" : "uri"
    } ],
    "http://linkedlifedata.com/resource/pubmed/owner" : [ {
      "value" : "NLM",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/lifeskim/mentions" : [ {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1882979",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0684224",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0557651",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C2347695",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1546471",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0596306",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0871208",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0678594",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0001721",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1552740",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0733755",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1314939",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1511726",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1513916",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0086168",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1880177",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C2936886",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0175168",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0005477",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0679932",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0012222",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1689985",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1704675",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1145667",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C2349975",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1883712",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1704735",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1510827",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C2348040",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1711351",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0030956",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0162340",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0022023",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1948059",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1521840",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0542560",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0182400",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1701901",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1709915",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0242210",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0596988",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0679209",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1883221",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0332128",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0332232",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1718271",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1149098",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1511695",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0677597",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1516144",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1707271",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1705682",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0079419",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1705526",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0597611",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C1419790",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0332290",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/umls/id/C0752218",
      "type" : "uri"
    } ],
    "http://linkedlifedata.com/resource/pubmed/chemical" : [ {
      "value" : "http://linkedlifedata.com/resource/pubmed/chemical/Calcium",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/chemical/Alanine",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/chemical/Glutamic+Acid",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular+Substances",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/chemical/Nerve+Growth+Factors",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/chemical/Peptide+Fragments",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/chemical/S-100+calcium-binding+protein+beta+subunit",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/chemical/S100+Proteins",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/chemical/Terbium",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/chemical/Tumor+Suppressor+Protein+p53",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/chemical/Tumor+Suppressor+Proteins",
      "type" : "uri"
    } ],
    "http://linkedlifedata.com/resource/pubmed/month" : [ {
      "value" : "May",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/journal" : [ {
      "value" : "http://linkedlifedata.com/resource/pubmed/journal/0370623",
      "type" : "uri"
    } ],
    "http://www.w3.org/1999/02/22-rdf-syntax-ns#type" : [ {
      "value" : "http://linkedlifedata.com/resource/pubmed/Citation",
      "type" : "uri"
    } ],
    "http://linkedlifedata.com/resource/pubmed/dateCreated" : [ {
      "value" : "2005-5-10",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/issue" : [ {
      "value" : "19",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/day" : [ {
      "value" : "17",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/affiliation" : [ {
      "value" : "Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 North Greene Street, Baltimore, Maryland 21201, USA.",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/publicationType" : [ {
      "value" : "Journal Article",
      "type" : "literal"
    }, {
      "value" : "Comparative Study",
      "type" : "literal"
    }, {
      "value" : "Research Support, N.I.H., Extramural",
      "type" : "literal"
    }, {
      "value" : "Research Support, Non-U.S. Gov't",
      "type" : "literal"
    }, {
      "value" : "Research Support, U.S. Gov't, P.H.S.",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/issnType" : [ {
      "value" : "Print",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/dateRevised" : [ {
      "value" : "2007-11-14",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/authorsComplete" : [ {
      "value" : "Y",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/author" : [ {
      "value" : "http://linkedlifedata.com/resource/pubmed/author/WeberDavid+JDJ",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/author/RustandiRichard+RRR",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/author/MarkowitzJosephJ",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/author/WilderPaul+TPT",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/author/UdanRyanR",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/author/VarneyKristen+MKM",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/author/WuSu+LingSL",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/author/HorrocksWilliam+DeWWD",
      "type" : "uri"
    } ],
    "http://linkedlifedata.com/resource/pubmed/status" : [ {
      "value" : "MEDLINE",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/year" : [ {
      "value" : "2005",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/volume" : [ {
      "value" : "44",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/citationSubset" : [ {
      "value" : "IM",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/articleTitle" : [ {
      "value" : "Calcium-binding properties of wild-type and EF-hand mutants of S100B in the presence and absence of a peptide derived from the C-terminal negative regulatory domain of p53.",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/meshHeading" : [ {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Alanine",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Amino+Acid+Sequence",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Animals",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Calcium",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-EF+Hand+Motifs",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Glutamic+Acid",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Humans",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Macromolecular+Substances",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Magnetic+Resonance+Spectroscopy",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Molecular+Sequence+Data",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Nerve+Growth+Factors",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Peptide+Fragments",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Protein+Binding",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Protein+Structure%2C+Tertiary",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Rats",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-S100+Proteins",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Terbium",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Tumor+Suppressor+Protein+p53",
      "type" : "uri"
    }, {
      "value" : "http://linkedlifedata.com/resource/pubmed/meshheading/15882069-Tumor+Suppressor+Proteins",
      "type" : "uri"
    } ],
    "http://linkedlifedata.com/resource/pubmed/pagination" : [ {
      "value" : "7305-14",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/issn" : [ {
      "value" : "0006-2960",
      "type" : "literal"
    } ],
    "http://linkedlifedata.com/resource/pubmed/language" : [ {
      "value" : "eng",
      "type" : "literal"
    } ],
    "http://www.w3.org/2004/02/skos/core#exactMatch" : [ {
      "value" : "http://purl.uniprot.org/pubmed/15882069",
      "type" : "uri"
    } ]
  }
}