Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-5-30
pubmed:abstractText
Transcription of the CA9 gene coding for a tumor-associated carbonic anhydrase IX (CA IX) isoform is regulated by hypoxia via the hypoxia-inducible factor 1 (HIF-1) and by high cell density via the phosphatidylinositol-3-kinase (PI3K) pathway. We examined the role of the mitogen-activated protein kinase (MAPK) pathway in the control of CA9 gene expression. Inhibition of MAPK signaling by U0126 in HeLa cells led to reduced activity of the PR1-HRE-luc CA9 promoter construct and decreased CA IX protein levels in dense culture as well as in hypoxia. Similar reduction was obtained by expression of a dominant-negative ERK1 mutant and was also observed in U0126-treated HIF-1alpha-deficient Ka13 cells. Simultaneous treatment with the MAPK and PI3K inhibitors U0126 and LY 294002 had stronger effect than individual inhibition of these pathways. Taken together, our results suggest that besides the PI3K pathway, the MAPK cascade is involved in the regulation of CA9 gene expression under both hypoxia and high cell density.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/2-(4-morpholinyl)-8-phenyl-4H-1-benz..., http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm, http://linkedlifedata.com/resource/pubmed/chemical/Butadienes, http://linkedlifedata.com/resource/pubmed/chemical/CA9 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Carbonic Anhydrases, http://linkedlifedata.com/resource/pubmed/chemical/Chromones, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/HIF1A protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Hypoxia-Inducible Factor 1, http://linkedlifedata.com/resource/pubmed/chemical/Hypoxia-Inducible Factor 1, alpha..., http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3, http://linkedlifedata.com/resource/pubmed/chemical/Morpholines, http://linkedlifedata.com/resource/pubmed/chemical/Nitriles, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/U 0126
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
1729
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
41-9
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:15833446-Antigens, Neoplasm, pubmed-meshheading:15833446-Butadienes, pubmed-meshheading:15833446-Carbonic Anhydrases, pubmed-meshheading:15833446-Cell Count, pubmed-meshheading:15833446-Cell Hypoxia, pubmed-meshheading:15833446-Chromones, pubmed-meshheading:15833446-DNA-Binding Proteins, pubmed-meshheading:15833446-Enzyme Inhibitors, pubmed-meshheading:15833446-Gene Expression Regulation, pubmed-meshheading:15833446-HeLa Cells, pubmed-meshheading:15833446-Humans, pubmed-meshheading:15833446-Hypoxia-Inducible Factor 1, pubmed-meshheading:15833446-Hypoxia-Inducible Factor 1, alpha Subunit, pubmed-meshheading:15833446-MAP Kinase Signaling System, pubmed-meshheading:15833446-Mitogen-Activated Protein Kinase 3, pubmed-meshheading:15833446-Morpholines, pubmed-meshheading:15833446-Nitriles, pubmed-meshheading:15833446-Nuclear Proteins, pubmed-meshheading:15833446-Phosphatidylinositol 3-Kinases, pubmed-meshheading:15833446-Transcription Factors
pubmed:year
2005
pubmed:articleTitle
MAPK pathway contributes to density- and hypoxia-induced expression of the tumor-associated carbonic anhydrase IX.
pubmed:affiliation
Centre of Molecular Medicine, Institute of Virology, Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, Slovakia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't