Linked Life Data

15748898

Source:http://linkedlifedata.com/resource/pubmed/id/15748898

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-3-7
pubmed:abstractText
Adipose tissue of HIV-1-infected patients shows severe abnormalities such as profound changes in adipose tissue morphology and metabolism. Does HIV-1 infect the adipose cell remains an unsolved question since previous attempts showed that HIV-1 poorly infects human adipocytes in vitro. In the present study, preadipose cells from human subcutaneous fat pads were differentiated in vitro, checked for HIV receptor expression, then infected with R5 and X4 HIV1 strains. Using a sensitive RT-PCR assay, we showed that HIV-1 tat and rev early viral transcripts were expressed in infected adipocytes giving a clear evidence of HIV-1 transcriptional activity in these cells. However, at the same time, no sign of productive infection was demonstrated since infected adipocytes did not efficiently produce Gag p24 antigen. We hypothesized that such a limitation could result from the lack of activation of adipocyte-signaling pathways able to stimulate HIV-1 gene expression in quiescent adipocytes. Indeed, a significant increase in Gag p24 production was observed after stimulation of infected adipocytes with pro-inflammatory cytokines, such as tumor necrosis factor alpha or interleukin-1-beta. Taken together, these results demonstrate that HIV-1 does infect human adipose cells in vitro and suggest that the initial limited infection can be overcome upon pro-inflammatory cytokine treatment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0014-4827
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
304
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
544-51
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15748898-Adipocytes, pubmed-meshheading:15748898-Cells, Cultured, pubmed-meshheading:15748898-Gene Expression Regulation, Viral, pubmed-meshheading:15748898-Gene Products, rev, pubmed-meshheading:15748898-Gene Products, tat, pubmed-meshheading:15748898-HIV Core Protein p24, pubmed-meshheading:15748898-HIV-1, pubmed-meshheading:15748898-HIV-Associated Lipodystrophy Syndrome, pubmed-meshheading:15748898-Humans, pubmed-meshheading:15748898-Inflammation Mediators, pubmed-meshheading:15748898-Signal Transduction, pubmed-meshheading:15748898-Transcriptional Activation, pubmed-meshheading:15748898-Tumor Necrosis Factor-alpha, pubmed-meshheading:15748898-Virus Replication, pubmed-meshheading:15748898-rev Gene Products, Human Immunodeficiency Virus, pubmed-meshheading:15748898-tat Gene Products, Human Immunodeficiency Virus
pubmed:year
2005
pubmed:articleTitle
Tumor necrosis factor-alpha stimulates HIV-1 production in primary culture of human adipocytes.
pubmed:affiliation
U526-Laboratoire de Virologie, Faculté de Médecine, Av. de Valombrose, 06107- NICE cedex 2, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't