Linked Life Data

15528251

Source:http://linkedlifedata.com/resource/pubmed/id/15528251

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-3-14
pubmed:abstractText
Heat shock proteins (HSP) 25 and 72 are expressed normally by surface colonocytes but not by small intestinal enterocytes. We hypothesized that luminal commensal microflora maintain the observed colonocyte HSP expression. The ability of the small intestine to respond to bacteria and their products and modulate HSPs has not been determined. The effects of luminal bacterial flora in surgically created midjejunal self-filling (SFL) vs. self-emptying (SEL) small-bowel blind loops on epithelial HSP expression were studied. HSP25 and HSP72 expression were assessed by immunoblot and immunohistochemistry. SFL were chronically colonized, whereas SEL contained levels of bacteria normal for the proximal small intestine. SFL creation significantly increased HSP25 and HSP72 expression relative to corresponding sections from SEL. Metronidazole treatment, which primarily affects anaerobic bacteria as well as a diet lacking fermentable fiber, significantly decreased SFL HSP expression. Small bowel incubation with butyrate ex vivo induced a sustained and significant upregulation of HSP25 and altered HSP72 expression, confirming the role of short-chain fatty acids. To determine whether HSPs induction altered responses to an injury, effects of the oxidant, monochloramine, on epithelial resistance and short-circuit current (I(sc)) responses to carbachol and glucose were compared. Increased SFL HSP expression was associated with protection against oxidant-induced decreases in transmural resistance and I(sc) responses to glucose, but not secretory responses to carbachol. In conclusion, luminal microflora and their metabolic byproducts direct expression of HSPs in gut epithelial cells, an effect that contributes to preservation of epithelial cell viability under conditions of stress.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0193-1857
pubmed:author
pubmed:issnType
Print
pubmed:volume
288
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
G696-704
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15528251-Animals, pubmed-meshheading:15528251-Bacterial Physiological Phenomena, pubmed-meshheading:15528251-Biological Transport, pubmed-meshheading:15528251-Butyrates, pubmed-meshheading:15528251-Chloramines, pubmed-meshheading:15528251-Cytoprotection, pubmed-meshheading:15528251-Dietary Fiber, pubmed-meshheading:15528251-Fatty Acids, Volatile, pubmed-meshheading:15528251-Fermentation, pubmed-meshheading:15528251-Gastrointestinal Contents, pubmed-meshheading:15528251-Gastrointestinal Tract, pubmed-meshheading:15528251-HSP27 Heat-Shock Proteins, pubmed-meshheading:15528251-HSP72 Heat-Shock Proteins, pubmed-meshheading:15528251-Heat-Shock Proteins, pubmed-meshheading:15528251-Intestinal Mucosa, pubmed-meshheading:15528251-Intestine, Small, pubmed-meshheading:15528251-Jejunum, pubmed-meshheading:15528251-Male, pubmed-meshheading:15528251-Neoplasm Proteins, pubmed-meshheading:15528251-Oxidants, pubmed-meshheading:15528251-Permeability, pubmed-meshheading:15528251-Rats, pubmed-meshheading:15528251-Rats, Sprague-Dawley, pubmed-meshheading:15528251-Tissue Distribution
pubmed:year
2005
pubmed:articleTitle
Luminal bacterial flora determines physiological expression of intestinal epithelial cytoprotective heat shock proteins 25 and 72.
pubmed:affiliation
Martin Boyer Professor of Medicine, The Univ. of Chicago, 5841 S. Maryland Ave. Rm. G705, MC 6084, Chicago, IL, 60637, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't