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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2004-9-13
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pubmed:abstractText |
We have established a transgenic mouse line in which floxed neomycin resistant cassette was inserted between the CAG promoter and EGFP. When these transgenic mice were mated with Cre-expressing transgenic animals, the offspring obtained were fluorescent green. We then established a transgenic mouse line in which EGFP in the above construct was replaced by diphtheria toxin A chain (DT). When the latter transgenic mice were mated with mice expressing Cre restricted to germ cells, we obtained healthy but sterile offspring due to a disruption of germ line cells by DT expression. We predict that this strategy will be useful for the construction of new animal models for human diseases, featuring a variety of missing cell lineages produced by disruption with DT.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
321
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
275-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:15358172-Animals,
pubmed-meshheading:15358172-Cell Lineage,
pubmed-meshheading:15358172-Diphtheria Toxin,
pubmed-meshheading:15358172-Disease Models, Animal,
pubmed-meshheading:15358172-Embryo, Mammalian,
pubmed-meshheading:15358172-Gene Expression,
pubmed-meshheading:15358172-Integrases,
pubmed-meshheading:15358172-Mice,
pubmed-meshheading:15358172-Mice, Transgenic,
pubmed-meshheading:15358172-Organ Size,
pubmed-meshheading:15358172-Organ Specificity,
pubmed-meshheading:15358172-Peptide Fragments,
pubmed-meshheading:15358172-Viral Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain.
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pubmed:affiliation |
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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