Linked Life Data

15251197

Source:http://linkedlifedata.com/resource/pubmed/id/15251197

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-7-14
pubmed:abstractText
A chloramphenicol acetyltransferase (catB7) gene containing two point mutations, 181A/G and 314A/G, has been recently reported to be a determinant for high-level chloramphenicol resistance phenotype in a Pseudomonas aeruginosa strain PAhcr1. The mutant CATB7 was further characterized in vitro and in vivo to elucidate the molecular basis of high-level resistance. CAT assay demonstrated that the mutant and wild-type recombinant CATB7 had similar specific activities. Dot blotting revealed that the accumulated amounts of CATB7 in P. aeruginosa strains PAO1 and PAhcr1 were proportionate to the respective anti-chloramphenicol level. Site-directed mutagenesis showed that G61S and Y105C contributed synergistically to the PAhcr1 resistance phenotype. It could be proposed that the mutant CATB7 was more structurally stable than catalytically efficient as a chloramphenicol resistance determinant in PAhcr1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0378-1097
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
236
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
197-204
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Mutations in the chloramphenicol acetyltransferase (S61G, Y105C) increase accumulated amounts and resistance in Pseudomonas aeruginosa.
pubmed:affiliation
School of Life Science & Technology, Shanghai Jiaotong University, No. 800 Dong-Chuan Road, Shanghai 200240, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't