Source:http://linkedlifedata.com/resource/pubmed/id/15173607
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2004-6-2
|
| pubmed:abstractText |
Two-dimensional electrophoresis (2-DE) is a powerful technique to differentially display patterns of protein expression and posttranslational modifications, providing a good strategy to monitor molecular responses induced by the activation or inactivation of specific signaling pathways. In this chapter, optimized protocols for 2-DE using extracts from tissue culture are provided. Protocols for in-gel digestion of gel-resolved proteins, which allow protein identification by mass spectrometry are also discussed.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
1064-3745
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
284
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
37-49
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:15173607-Cell Line, Tumor,
pubmed-meshheading:15173607-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:15173607-Humans,
pubmed-meshheading:15173607-Isoelectric Focusing,
pubmed-meshheading:15173607-Mass Spectrometry,
pubmed-meshheading:15173607-Proteins,
pubmed-meshheading:15173607-Signal Transduction,
pubmed-meshheading:15173607-Silver Staining
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
Two-dimensional gel electrophoresis for the identification of signaling targets.
|
| pubmed:affiliation |
Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado at Boulder, USA.
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| pubmed:publicationType |
Journal Article
|