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pubmed-article:14738938pubmed:abstractTextA simple high performance liquid chromatography (HPLC) method was developed for the determination of butein in rat serum. The method involved the deproteinization followed by injection into a Luna C8 column. Butein was eluted at 3.8 min at a flow rate of 0.2 ml/min with the mobile phase of acetonitrile-ammonium formate (10mM, pH 3.0) (35:65, v/v). The standard curve was linear (r(2)=0.995) over the concentration range of 0.1-10 microg/ml. The coefficient of variation (CV) of intra- and inter-assay ranged from 2.7 to 7.5% and 6.0 to 7.5%, respectively. The limit of quantification was 0.1 microg/ml using a serum sample of 50 microl. This method was applied to a pharmacokinetic study after intravenous injection of butein (5mg/kg) to rats.lld:pubmed
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pubmed-article:14738938pubmed:authorpubmed-author:KimYoun-ChulY...lld:pubmed
pubmed-article:14738938pubmed:authorpubmed-author:SohnDong...lld:pubmed
pubmed-article:14738938pubmed:authorpubmed-author:LeeHye WonHWlld:pubmed
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pubmed-article:14738938pubmed:authorpubmed-author:JiHye YoungHYlld:pubmed
pubmed-article:14738938pubmed:authorpubmed-author:ChungHun...lld:pubmed
pubmed-article:14738938pubmed:authorpubmed-author:LeeHong IlHIlld:pubmed
pubmed-article:14738938pubmed:authorpubmed-author:KimHae-Kyoung...lld:pubmed
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pubmed-article:14738938pubmed:pagination227-31lld:pubmed
pubmed-article:14738938pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:14738938pubmed:year2004lld:pubmed
pubmed-article:14738938pubmed:articleTitleDetermination of butein in rat serum by high performance liquid chromatography.lld:pubmed
pubmed-article:14738938pubmed:affiliationCollege of Pharmacy and Medicinal Resources Research Center, Wonkwang University, Shinyongdong, Iksan 570-749, South Korea.lld:pubmed
pubmed-article:14738938pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:14738938pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed