Source:http://linkedlifedata.com/resource/pubmed/id/14518312
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
Suppl I
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pubmed:dateCreated |
2003-10-1
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pubmed:abstractText |
Up to this day, the influence of laparoscopy and hyperbaric carbon dioxide on the growth of tumor cells is not yet clear. Cells of human colonic carcinoma CX-2 and pancreatic carcinoma DAN-G were exposed to carbon dioxide under pressures of zero, 6 and 12 mmHg. Proliferation was measured by counting in NEUBAUER's counting chamber and by DNAstaining with Pico Green over five days after exposition. In a third experiment, dead cells were stained with Propidium Iodide and detected by flow cytometry. Only DAN-G after 6 mmHg showed at day five an increasing proliferation rate. Furthermore, exposition to carbon dioxide lead to an increased necrosis of cells. All other groups showed a significantly decreased proliferating activity of the cells exposed to carbon dioxide in comparison to a control group.
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pubmed:language |
ger
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0942-2854
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
115
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
535-40
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pubmed:dateRevised |
2008-2-20
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pubmed:meshHeading |
pubmed-meshheading:14518312-Air Pressure,
pubmed-meshheading:14518312-Carbon Dioxide,
pubmed-meshheading:14518312-Cell Count,
pubmed-meshheading:14518312-Cell Division,
pubmed-meshheading:14518312-Cell Survival,
pubmed-meshheading:14518312-Colonic Neoplasms,
pubmed-meshheading:14518312-Flow Cytometry,
pubmed-meshheading:14518312-Humans,
pubmed-meshheading:14518312-Pancreatic Neoplasms,
pubmed-meshheading:14518312-Pneumoperitoneum, Artificial,
pubmed-meshheading:14518312-Tumor Cells, Cultured
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pubmed:year |
1998
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pubmed:articleTitle |
[CO2 pneumoperitoneum inhibits in vitro proliferation of human carcinoma cells].
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pubmed:affiliation |
Klinik für Allgemein- und Gefässchirurgie, Johann Wolfgang Goethe-Universität, Frankfurt am Main. Gutt@em.uni-frankfurt.de
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pubmed:publicationType |
Journal Article,
Comparative Study,
English Abstract
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