pubmed-article:1360647 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:1360647 | lifeskim:mentions | umls-concept:C0013935 | lld:lifeskim |
pubmed-article:1360647 | lifeskim:mentions | umls-concept:C0012892 | lld:lifeskim |
pubmed-article:1360647 | lifeskim:mentions | umls-concept:C0013139 | lld:lifeskim |
pubmed-article:1360647 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
pubmed-article:1360647 | pubmed:issue | 21 | lld:pubmed |
pubmed-article:1360647 | pubmed:dateCreated | 1993-1-4 | lld:pubmed |
pubmed-article:1360647 | pubmed:abstractText | Genetic and biochemical evidence suggests there are at least three DNA polymerases required for replication in eukaryotic cells. However, Drosophila embryonic cells have a very short duration S phase which is regulated differently. To address the question of whether embryos utilize different DNA polymerases, we employed Mono Q anion exchange chromatography to resolve the DNA polymerase activities. Two types of DNA polymerase, DNA polymerase delta and DNA polymerase alpha, were distinguished by: 1. copurification of DNA primase or 3'-5'exonuclease activities; 2. immunoblot analysis with alpha-specific polyclonal antisera; 3. sensitivity to aphidicolin and BuPdGTP; and 4. processivity measurements with and without Proliferating Cell Nuclear Antigen. These observations suggest that Drosophila embryos, similar to nonembryonic cells, have both alpha- and delta-type DNA polymerases. | lld:pubmed |
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pubmed-article:1360647 | pubmed:language | eng | lld:pubmed |
pubmed-article:1360647 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1360647 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:1360647 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:1360647 | pubmed:month | Nov | lld:pubmed |
pubmed-article:1360647 | pubmed:issn | 0305-1048 | lld:pubmed |
pubmed-article:1360647 | pubmed:author | pubmed-author:GernerE WEW | lld:pubmed |
pubmed-article:1360647 | pubmed:author | pubmed-author:CressA EAE | lld:pubmed |
pubmed-article:1360647 | pubmed:author | pubmed-author:PeckV MVM | lld:pubmed |
pubmed-article:1360647 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:1360647 | pubmed:day | 11 | lld:pubmed |
pubmed-article:1360647 | pubmed:volume | 20 | lld:pubmed |
pubmed-article:1360647 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:1360647 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:1360647 | pubmed:pagination | 5779-84 | lld:pubmed |
pubmed-article:1360647 | pubmed:dateRevised | 2010-9-7 | lld:pubmed |
pubmed-article:1360647 | pubmed:meshHeading | pubmed-meshheading:1360647-... | lld:pubmed |
pubmed-article:1360647 | pubmed:meshHeading | pubmed-meshheading:1360647-... | lld:pubmed |
pubmed-article:1360647 | pubmed:meshHeading | pubmed-meshheading:1360647-... | lld:pubmed |
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pubmed-article:1360647 | pubmed:meshHeading | pubmed-meshheading:1360647-... | lld:pubmed |
pubmed-article:1360647 | pubmed:meshHeading | pubmed-meshheading:1360647-... | lld:pubmed |
pubmed-article:1360647 | pubmed:meshHeading | pubmed-meshheading:1360647-... | lld:pubmed |
pubmed-article:1360647 | pubmed:meshHeading | pubmed-meshheading:1360647-... | lld:pubmed |
pubmed-article:1360647 | pubmed:year | 1992 | lld:pubmed |
pubmed-article:1360647 | pubmed:articleTitle | Delta-type DNA polymerase characterized from Drosophila melanogaster embryos. | lld:pubmed |
pubmed-article:1360647 | pubmed:affiliation | Department of Radiation Oncology, Arizona Cancer Center, University of Arizona, Tucson 85724. | lld:pubmed |
pubmed-article:1360647 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:1360647 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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