Linked Life Data

1356158

Source:http://linkedlifedata.com/resource/pubmed/id/1356158

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1992-10-19
pubmed:abstractText
Astrocyte-enriched cultures were established upon passaging of primary cultures from the myelencephalon and mesencephalon of 7-9-week-old human embryos. Immunocytochemical analysis showed that third-fourth passage cultures were composed of a highly enriched population of proliferating, epithelioid cells, up to 90% of which expressed glial fibrillary acidic protein (GFAP); no macrophages and very few fibroblasts (less than 2%) were present. GFAP expression and proliferation declined upon further culturing in serum-containing medium but could be transiently reinduced by growing the cells in a serum-free chemically defined medium. Large numbers of GFAP+ astrocytes were obtained from each embryo and could be stored frozen and recultured. Using flow cytometric analysis, human astrocyte cultures were examined for basal and cytokine [interferon-gamma (IFN-gamma), interleukin-1 beta (IL-1 beta), and tumor necrosis factor-alpha (TNF-alpha)]-induced expression of molecules that may be involved in astrocyte-T-lymphocyte interactions. Cultured human astrocytes spontaneously expressed major histocompatibility complex (MHC) class I antigens and variable levels of MHC class II; MHC class I levels were increased upon IFN-gamma and TNF-alpha treatment, whereas MHC class II antigens were induced on most of the astrocytes by IFN-gamma. Among the molecules involved in antigen-independent interactions between T lymphocytes and target cells, lymphocyte function-associated molecule-3 (LFA-3) was spontaneously expressed by most cultured human astrocytes, whereas intercellular adhesion molecule-1 (ICAM-1) was present at variable levels in non-stimulated astrocytes and was greatly induced by IFN-gamma, TNF-alpha, and IL-1 beta. In this study we also show that the above cytokines upregulate astroglial expression of adhesion molecules of the integrin family (VLA-1, VLA-2, and VLA-6) that may be involved in astrocyte-extracellular matrix interaction and play a role in the astrocyte reactive changes occurring at sites of brain injury and inflammation. The human astrocyte cultures developed here represent a useful in vitro model to further investigate mechanisms involved in bidirectional communication between central glia and cells of the immune system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0360-4012
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
494-506
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:1356158-Astrocytes, pubmed-meshheading:1356158-Brain, pubmed-meshheading:1356158-Cell Adhesion Molecules, pubmed-meshheading:1356158-Cytokines, pubmed-meshheading:1356158-Embryo, Mammalian, pubmed-meshheading:1356158-Female, pubmed-meshheading:1356158-Fibronectins, pubmed-meshheading:1356158-Flow Cytometry, pubmed-meshheading:1356158-Humans, pubmed-meshheading:1356158-Immunohistochemistry, pubmed-meshheading:1356158-Inflammation, pubmed-meshheading:1356158-Integrins, pubmed-meshheading:1356158-Intercellular Adhesion Molecule-1, pubmed-meshheading:1356158-Lymphocyte Function-Associated Antigen-1, pubmed-meshheading:1356158-Macrophages, pubmed-meshheading:1356158-Major Histocompatibility Complex, pubmed-meshheading:1356158-Neuroglia, pubmed-meshheading:1356158-Pregnancy, pubmed-meshheading:1356158-Receptors, Very Late Antigen
pubmed:year
1992
pubmed:articleTitle
Astrocyte cultures from human embryonic brain: characterization and modulation of surface molecules by inflammatory cytokines.
pubmed:affiliation
Neurobiology Section, Istituto Superiore di Sanità, Rome, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't