| pubmed-article:12700958 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12700958 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:12700958 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:12700958 | lifeskim:mentions | umls-concept:C0022677 | lld:lifeskim |
| pubmed-article:12700958 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
| pubmed-article:12700958 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:12700958 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:12700958 | pubmed:dateCreated | 2003-4-17 | lld:pubmed |
| pubmed-article:12700958 | pubmed:abstractText | Macrophages play important roles during renal inflammation. Infiltrating macrophages produce proinflammatory mediators and induce apoptosis in a variety of target cells. Because proximal tubules are frequently damaged in inflammatory processes, we investigated murine macrophages (J774) in the induction of apoptosis in murine PKSV-PR proximal tubule cells. PKSV-PR cells were co-cultured with activated or non-activated macrophages. Apoptosis was assessed by Annexin-V-FITC/propidium iodide staining and analyzed by fluorescence-activated cell sorting. Macrophages were separated from tubule cells with transwell membranes to distinguish soluble factor-mediated from direct cell-to-cell contact-mediated apoptosis. Cell supernatants from activated and non-activated macrophages were analyzed for induction of apoptosis. Activated (but not non-activated) macrophages induced tubule cell apoptosis in co-culture. Soluble factors were mainly responsible for induction of apoptosis; membrane separation and transfer of cell supernatant from activated macrophages showed similar levels of apoptosis induction. Although tumor necrosis factor (TNF)-alpha and transforming growth factor (TGF)-beta(1), measured by ELISA, increased significantly in supernatants from activated macrophages, blocking TNF-alpha and TGF-beta did not decrease apoptosis in PKSV-PR cells co-cultured with macrophages. Moreover, exogenous addition of TNF-alpha, TGF-beta, anti-Fas antibody, or TRAIL failed to induce apoptosis in tubule cells. We conclude that inflammatory macrophages mediate proximal tubule cell death, directing apoptosis mainly via release of unidentified soluble factors. | lld:pubmed |
| pubmed-article:12700958 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700958 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700958 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12700958 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700958 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12700958 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12700958 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700958 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700958 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700958 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700958 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12700958 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12700958 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:12700958 | pubmed:issn | 0931-041X | lld:pubmed |
| pubmed-article:12700958 | pubmed:author | pubmed-author:VandewalleAla... | lld:pubmed |
| pubmed-article:12700958 | pubmed:author | pubmed-author:Lange-Sperand... | lld:pubmed |
| pubmed-article:12700958 | pubmed:author | pubmed-author:ChevalierRobe... | lld:pubmed |
| pubmed-article:12700958 | pubmed:author | pubmed-author:FuldaSimoneS | lld:pubmed |
| pubmed-article:12700958 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12700958 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:12700958 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12700958 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12700958 | pubmed:pagination | 335-41 | lld:pubmed |
| pubmed-article:12700958 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:12700958 | pubmed:meshHeading | pubmed-meshheading:12700958... | lld:pubmed |
| pubmed-article:12700958 | pubmed:meshHeading | pubmed-meshheading:12700958... | lld:pubmed |
| pubmed-article:12700958 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12700958 | pubmed:articleTitle | Macrophages induce apoptosis in proximal tubule cells. | lld:pubmed |
| pubmed-article:12700958 | pubmed:affiliation | Department of Pediatrics, University of Virginia, VA 22908, Charlottesville, USA. | lld:pubmed |
| pubmed-article:12700958 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12700958 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:12700958 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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