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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2002-7-9
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pubmed:abstractText |
DNA topoisomerase IIalpha (Topo IIalpha) is regulated in late S phase-dependent manner. To identify late S phase-dependent cis-acting elements of Topo IIalpha gene, we have investigated the synchronized HeLa cells with chloramphenicol acetyltransferase and DNase I footprinting assays. The level of Topo IIalpha mRNA increased after release from aphidicolin block and reached a maximum in 8h (late S phase) in HeLa cells, and Topo II unknotting activity was also in parallel with the level of Topo IIalpha mRNA. The late S phase-regulatory element was found to be located in the region containing ATF-binding element between -290 and -90bp and the region was required for a maximal stimulation during late S phase. DNase I footprinting assay showed that ATF-binding element and novel cis-acting element (Topo IIalpha-specific sequence) were the principal protein-binding sites and the proteins interacting with these elements were induced during late S phase. One DNA-protein complex was formed by DNA mobility shift assay when ATF-binding site was incubated with nuclear extract prepared from late S phase cells, but no protein bound in non-S phase cells. Taken together, these results suggest that ATF may be essential transacting factor for maximal expression of Topo IIalpha gene during late S phase in HeLa cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Activating Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Topoisomerases, Type II,
http://linkedlifedata.com/resource/pubmed/chemical/DNA topoisomerase II alpha,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease I,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0304-3835
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pubmed:author |
pubmed-author:AhnJong-WoongJW,
pubmed-author:HwangByung-DooBD,
pubmed-author:JoeL KLK,
pubmed-author:KimTae-JeongTJ,
pubmed-author:KweonKwang-InKI,
pubmed-author:LeeYoung-ChulYC,
pubmed-author:NoZaesungZ,
pubmed-author:ParkChungC,
pubmed-author:ParkJong-IlJI,
pubmed-author:ParkSeung-KielSK,
pubmed-author:SonMee-YoungMY,
pubmed-author:YoonWan-HeeWH
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
184
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
81-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:12104051-Activating Transcription Factors,
pubmed-meshheading:12104051-Antigens, Neoplasm,
pubmed-meshheading:12104051-Base Sequence,
pubmed-meshheading:12104051-Blood Proteins,
pubmed-meshheading:12104051-Blotting, Northern,
pubmed-meshheading:12104051-Cell Cycle,
pubmed-meshheading:12104051-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:12104051-DNA Footprinting,
pubmed-meshheading:12104051-DNA Topoisomerases, Type II,
pubmed-meshheading:12104051-DNA-Binding Proteins,
pubmed-meshheading:12104051-Deoxyribonuclease I,
pubmed-meshheading:12104051-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:12104051-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:12104051-Genes, Regulator,
pubmed-meshheading:12104051-HeLa Cells,
pubmed-meshheading:12104051-Humans,
pubmed-meshheading:12104051-Molecular Sequence Data,
pubmed-meshheading:12104051-Neoplasm Proteins,
pubmed-meshheading:12104051-Promoter Regions, Genetic,
pubmed-meshheading:12104051-RNA, Messenger,
pubmed-meshheading:12104051-S Phase,
pubmed-meshheading:12104051-Transcription, Genetic,
pubmed-meshheading:12104051-Transcription Factors,
pubmed-meshheading:12104051-Transcriptional Activation
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pubmed:year |
2002
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pubmed:articleTitle |
ATF is important to late S phase-dependent regulation of DNA topoisomerase IIalpha gene expression in HeLa cells.
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pubmed:affiliation |
Department of Biochemistry, Chungnam National University, Moonhwa-dong, Jungu, Daejeon 301-747, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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