|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1-3
|
|
pubmed:dateCreated |
2002-4-17
|
|
pubmed:abstractText |
Human immunodeficiency virus type-1 envelope glycoprotein (gp120(env)) binding to cell surface CD4 receptor triggers a broad range of intracellular effects leading to T cell activation and cell cycle entry. Among these effects we and others previously reported on the nuclear translocation of the nuclear factor-kappaB (NF-kappaB) transcription factor. The present work further investigates the signal transduction pathways involved in gp120(env)-induced NF-kappaB activation. We demonstrate that gp120(env)-CD4 interaction stimulates the hyperphosphorylation of IkappaB-alpha inhibitory protein. Conversely, overexpression of a dominant-negative IkappaB-alpha transgene mutated at S32 and S36 residues, abolishes gp120(env)-induced NF-kappaB activation. IkappaB kinases (IKKs) activity was found to be selectively enhanced following CD4 engagement with gp120(env) and to mediate the phosphorylation of IkappaB-alpha while co-transfection experiments using dominant-negative forms of IKKs inhibited gp120(env)-induced NF-kappaB activation. Taken together, these results confirm that IKKs complex play a key role in gp120(env)-induced NF-kappaB activation.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/CHUK protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp120,
http://linkedlifedata.com/resource/pubmed/chemical/I kappa B beta protein,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/IKBKB protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/IKBKE protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappaB inhibitor alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0014-5793
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
10
|
|
pubmed:volume |
516
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
257-64
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:11959143-Antigens, CD4,
pubmed-meshheading:11959143-CD4-Positive T-Lymphocytes,
pubmed-meshheading:11959143-DNA-Binding Proteins,
pubmed-meshheading:11959143-Genes, Reporter,
pubmed-meshheading:11959143-HIV Envelope Protein gp120,
pubmed-meshheading:11959143-HIV Long Terminal Repeat,
pubmed-meshheading:11959143-HIV-1,
pubmed-meshheading:11959143-HeLa Cells,
pubmed-meshheading:11959143-Humans,
pubmed-meshheading:11959143-I-kappa B Kinase,
pubmed-meshheading:11959143-I-kappa B Proteins,
pubmed-meshheading:11959143-NF-kappa B,
pubmed-meshheading:11959143-Phosphorylation,
pubmed-meshheading:11959143-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11959143-Signal Transduction,
pubmed-meshheading:11959143-Transcriptional Activation,
pubmed-meshheading:11959143-Transfection
|
|
pubmed:year |
2002
|
|
pubmed:articleTitle |
NF-kappaB activation upon interaction of HIV-1 envelope glycoproteins with cell surface CD4 involves IkappaB kinases.
|
|
pubmed:affiliation |
Laboratoire Infections Rétrovirales et Signalisation Cellulaire, CNRS UMR 5121, Institut de Biologie, 4 Bd Henri IV, 34060, Montpellier, France.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
