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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2002-3-8
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pubmed:databankReference |
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pubmed:abstractText |
GIRK2 (G protein-gated inwardly rectifying K(+) channel 2) located on the Down syndrome region 21q22.2 in humans has been reported to have several alternative transcripts and transcripts longer than 4 kb that do not have the poly-A tail. We sequenced GIRK2 transcripts with a long 3'-untranslated region (3'-UTR) containing multiple adenylate uridylate-rich elements (AREs) with the poly-A tail. In a 16-kb transcript, 28 AUUUA pentanucleotides, 9 AUUUUA hexanucleotides, 5 AUUUUUA heptanucleotides, and 3 UUAUUUA[U/A][U/A] nonanucleotides were found. Northern blot and in situ hybridization revealed abundant expression of the 16-kb transcripts in the rat brain despite no detectable signals in other tissues examined. The AREs have been reported to mediate the turnover of mRNAs encoding proteins regulating cellular proliferation/differentiation and body response to inflammatory and environmental stimuli. This is the first study indicating that ion channel transcripts have multiple AREs.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
(C)2002 Elsevier Science (USA).
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
291
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1265-71
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11883954-3' Untranslated Regions,
pubmed-meshheading:11883954-Animals,
pubmed-meshheading:11883954-Brain,
pubmed-meshheading:11883954-Cloning, Molecular,
pubmed-meshheading:11883954-DNA, Complementary,
pubmed-meshheading:11883954-G Protein-Coupled Inwardly-Rectifying Potassium Channels,
pubmed-meshheading:11883954-In Situ Hybridization,
pubmed-meshheading:11883954-Male,
pubmed-meshheading:11883954-Molecular Sequence Data,
pubmed-meshheading:11883954-Molecular Weight,
pubmed-meshheading:11883954-Poly A,
pubmed-meshheading:11883954-Potassium Channels,
pubmed-meshheading:11883954-Potassium Channels, Inwardly Rectifying,
pubmed-meshheading:11883954-RNA, Messenger,
pubmed-meshheading:11883954-Rats,
pubmed-meshheading:11883954-Rats, Sprague-Dawley,
pubmed-meshheading:11883954-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:11883954-Tissue Distribution
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pubmed:year |
2002
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pubmed:articleTitle |
Potassium channel mRNAs with AU-rich elements and brain-specific expression.
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pubmed:affiliation |
Department of Psychiatry, Jichi Medical School, Yakushiji 3311-1, Minamikawachi, Tochigi 239-0498, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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