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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2002-1-31
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pubmed:abstractText |
BCL6 translocation affecting the chromosomal band 3q27 can involve a number of non-immunoglobulin (non-IG) gene loci as partners. We report here that the gene for interleukin-21 receptor (IL-21R) is the partner of BCL6 in t(3;16)(q27;p11) translocation. The two breakpoints on 16p11 of a lymphoma cell line YM and case no. 1012 with diffuse large B-cell lymphoma, both of which carried t(3;16), were localized within the 27-kb intron 1 of IL-21R. As a result of t(3;16), the promoter region of IL-21R was substituted for the regulatory sequences of BCL6 in the same transcriptional orientation. Reverse transcriptase-mediated polymerase chain reaction revealed chimeric mRNA consisting of two non-coding exons 1a/1b of IL-21R and coding exons of BCL6 in both lymphoma cells. Fluorescence in situ chromosomal hybridization of YM metaphase cells revealed fusion signals that contained both the BCL6 and IL-21R sequences on the der(3)t(3;16) chromosome. IL-21R was actively transcribed in YM cells, while BCL6 that was under the control of the IL-21R promoter was only moderately expressed at the mRNA and protein level. We constructed expression plasmid of BCL6 that followed the promoter sequences of IL-21R. COS-7 cells transiently transfected with the plasmid expressed high level Bcl-6 protein and displayed nuclear staining with a characteristic punctate pattern by immunofluorescence, indicating that expression of BCL6 can be enhanced by t(3;16). This study added to the list of non-IG partners of BCL6 translocations a new class of gene, i.e. cytokine receptor gene, the expression of which is closely associated with lymphoid cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/IL21R protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-21 Receptor alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-6,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-21,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
368-76
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11821949-Animals,
pubmed-meshheading:11821949-Artificial Gene Fusion,
pubmed-meshheading:11821949-Base Sequence,
pubmed-meshheading:11821949-Cell Line,
pubmed-meshheading:11821949-Chromosome Breakage,
pubmed-meshheading:11821949-Chromosome Mapping,
pubmed-meshheading:11821949-Chromosomes, Human, Pair 16,
pubmed-meshheading:11821949-Chromosomes, Human, Pair 3,
pubmed-meshheading:11821949-DNA-Binding Proteins,
pubmed-meshheading:11821949-Exons,
pubmed-meshheading:11821949-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:11821949-Genes, Reporter,
pubmed-meshheading:11821949-Humans,
pubmed-meshheading:11821949-In Situ Hybridization, Fluorescence,
pubmed-meshheading:11821949-Interleukin-21 Receptor alpha Subunit,
pubmed-meshheading:11821949-Introns,
pubmed-meshheading:11821949-Jurkat Cells,
pubmed-meshheading:11821949-Luciferases,
pubmed-meshheading:11821949-Lymphoma, B-Cell,
pubmed-meshheading:11821949-Lymphoma, Large B-Cell, Diffuse,
pubmed-meshheading:11821949-Microscopy, Fluorescence,
pubmed-meshheading:11821949-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:11821949-Promoter Regions, Genetic,
pubmed-meshheading:11821949-Proto-Oncogene Proteins,
pubmed-meshheading:11821949-Proto-Oncogene Proteins c-bcl-6,
pubmed-meshheading:11821949-RNA, Messenger,
pubmed-meshheading:11821949-Receptors, Interleukin,
pubmed-meshheading:11821949-Receptors, Interleukin-21,
pubmed-meshheading:11821949-Transcription Factors,
pubmed-meshheading:11821949-Translocation, Genetic
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pubmed:year |
2002
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pubmed:articleTitle |
The gene for interleukin-21 receptor is the partner of BCL6 in t(3;16)(q27;p11), which is recurrently observed in diffuse large B-cell lymphoma.
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pubmed:affiliation |
First Division, Department of Internal Medicine, Faculty of Medicine, Kyoto University, 54 Shogoin-Kawaramachi, Sakyo-ku, Kyoto 606-8507, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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