Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2002-1-10
pubmed:abstractText
Gene therapy with the complementary DNA (cDNA) of the angiogenic cytokine vascular endothelial growth factor (VEGF) has emerged as a promising strategy in the treatment of myocardial and lower-limb ischaemia. The objective of this study was to determine whether these principles could be applied to a recognised model of skin-flap ischaemia. Plasmid vectors including the cDNA of green fluorescent protein (GFP) and one of three VEGF isoforms (A165, B167 or B186) were constructed, and their base sequences confirmed. GFP expression was used as a marker of successful in vitro transfection of human endothelial cells with each plasmid. The plasmids were then administered subcutaneously to rat abdominal skin flaps surgically rendered ischaemic, and the percentage of viable tissue was assessed at 1 week. Angiograms of the flaps and histological preparations of flap tissue were assessed for evidence of angiogenesis. The survival of flaps treated with VEGF A165 or B167 cDNA was significantly greater than that of controls (P < 0.05). The survival of flaps treated with VEGF B186 cDNA was greater than that of controls, but statistical significance was not reached. Angiograms and microvessel density counts failed to produce evidence of angiogenesis. With improved delivery strategies, VEGF may have a role in the management of surgical ischaemia.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0007-1226
pubmed:author
pubmed:copyrightInfo
Copyright 2002 The British Association of Plastic Surgeons.
pubmed:issnType
Print
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
55-8
pubmed:dateRevised
2006-5-1
pubmed:meshHeading
pubmed-meshheading:11783969-Analysis of Variance, pubmed-meshheading:11783969-Animals, pubmed-meshheading:11783969-DNA, Complementary, pubmed-meshheading:11783969-Endothelial Growth Factors, pubmed-meshheading:11783969-Gene Therapy, pubmed-meshheading:11783969-Green Fluorescent Proteins, pubmed-meshheading:11783969-Ischemia, pubmed-meshheading:11783969-Luminescent Proteins, pubmed-meshheading:11783969-Lymphokines, pubmed-meshheading:11783969-Male, pubmed-meshheading:11783969-Plasmids, pubmed-meshheading:11783969-Protein Isoforms, pubmed-meshheading:11783969-Rats, pubmed-meshheading:11783969-Rats, Sprague-Dawley, pubmed-meshheading:11783969-Surgical Flaps, pubmed-meshheading:11783969-Tissue Survival, pubmed-meshheading:11783969-Transfection, pubmed-meshheading:11783969-Vascular Endothelial Growth Factor A, pubmed-meshheading:11783969-Vascular Endothelial Growth Factors
pubmed:year
2002
pubmed:articleTitle
Vascular endothelial growth factor gene therapy in ischaemic rat skin flaps.
pubmed:affiliation
Department of Plastic and Reconstructive Surgery, St George Hospital, Sydney, New South Wales, Australia.
pubmed:publicationType
Journal Article