| pubmed-article:11724580 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11724580 | lifeskim:mentions | umls-concept:C0212694 | lld:lifeskim |
| pubmed-article:11724580 | lifeskim:mentions | umls-concept:C0012860 | lld:lifeskim |
| pubmed-article:11724580 | lifeskim:mentions | umls-concept:C0165675 | lld:lifeskim |
| pubmed-article:11724580 | lifeskim:mentions | umls-concept:C0542341 | lld:lifeskim |
| pubmed-article:11724580 | lifeskim:mentions | umls-concept:C1274040 | lld:lifeskim |
| pubmed-article:11724580 | lifeskim:mentions | umls-concept:C1511692 | lld:lifeskim |
| pubmed-article:11724580 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:11724580 | lifeskim:mentions | umls-concept:C1517945 | lld:lifeskim |
| pubmed-article:11724580 | lifeskim:mentions | umls-concept:C0054362 | lld:lifeskim |
| pubmed-article:11724580 | pubmed:issue | 48 | lld:pubmed |
| pubmed-article:11724580 | pubmed:dateCreated | 2001-11-28 | lld:pubmed |
| pubmed-article:11724580 | pubmed:abstractText | Treatment of cells with the enediyne C-1027 is highly efficient at inducing single- and double-strand DNA breaks. This agent is highly cytotoxic when used at picomolar levels over a period of days. For this study, C-1027 has been used at higher levels for a much shorter time period to look at early cellular responses to DNA strand breaks. Extracts from cells treated with C-1027 for as little as 2 h are deficient in SV40 DNA replication activity. Treatment with low levels of C-1027 (1-3 nM) does not result in the presence of a replication inhibitor in cell extracts, but they are deficient in replication protein A (RPA) function. Extracts from cells treated with high levels of C-1027 (10 nM) do show the presence of a trans-acting inhibitor of DNA replication. The deficiency in RPA in extracts from cells treated with low levels of C-1027 can be fully complemented by the addition of exogenous RPA, and may be due to a C-1027-induced decrease in the extractability of RPA. This decrease in the extractability of RPA correlates with the appearance of many extraction-resistant intranuclear RPA foci. The trans-acting inhibitor of DNA replication induced by treatment of cells with high levels of C-1027 (10 nM) is DNA-dependent protein kinase (DNA-PK). DNA-PK is activated by the presence of DNA fragments induced by C-1027 treatment, and can be abrogated by removal of the DNA fragments. Although it is activated by DNA damage and phosphorylates RPA, DNA-PK is not required for either RPA focalization or loss of RPA replication activity. | lld:pubmed |
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| pubmed-article:11724580 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11724580 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11724580 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11724580 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11724580 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11724580 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:11724580 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:11724580 | pubmed:author | pubmed-author:BeermanT ATA | lld:pubmed |
| pubmed-article:11724580 | pubmed:author | pubmed-author:LimJ KJK | lld:pubmed |
| pubmed-article:11724580 | pubmed:author | pubmed-author:HoY HYH | lld:pubmed |
| pubmed-article:11724580 | pubmed:author | pubmed-author:KwaS BSB | lld:pubmed |
| pubmed-article:11724580 | pubmed:author | pubmed-author:MelendyTT | lld:pubmed |
| pubmed-article:11724580 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11724580 | pubmed:day | 4 | lld:pubmed |
| pubmed-article:11724580 | pubmed:volume | 40 | lld:pubmed |
| pubmed-article:11724580 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11724580 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11724580 | pubmed:pagination | 14661-8 | lld:pubmed |
| pubmed-article:11724580 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:11724580 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11724580 | pubmed:articleTitle | DNA damage by the enediyne C-1027 results in the inhibition of DNA replication by loss of replication protein A function and activation of DNA-dependent protein kinase. | lld:pubmed |
| pubmed-article:11724580 | pubmed:affiliation | Department of Microbiology & Biochemistry, State University of New York at Buffalo, School of Medicine & Biomedical Sciences, Buffalo, New York 14214, USA. | lld:pubmed |
| pubmed-article:11724580 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11724580 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11724580 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:11724580 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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