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pubmed:abstractText |
Human trophoblast cells in primary culture are difficult to use for the rigorous study of trophoblast function because of contamination with other cell types, paucity of numbers, poor viability and inter-experiment variation engendered by the need to prepare fresh cells for each experiment. DNA-transfection to produce immortalized cells, or cells with extended life-span, has been the obvious approach to solve this problem. Although there have been a few reports in the literature describing trophoblast cell lines generated in this way, it is clear that to date the methods are difficult and few lines have been generated. The basic problem is that transfection efficiencies of different methods are cell type-specific. The objectives of this study were therefore to compare the transfection efficiencies of three commonly used techniques, to use the best technique to generate trophoblast cell lines, and to conduct preliminary characterization studies.
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