| pubmed-article:11710576 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11710576 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:11710576 | lifeskim:mentions | umls-concept:C0032105 | lld:lifeskim |
| pubmed-article:11710576 | lifeskim:mentions | umls-concept:C0302908 | lld:lifeskim |
| pubmed-article:11710576 | lifeskim:mentions | umls-concept:C0006812 | lld:lifeskim |
| pubmed-article:11710576 | lifeskim:mentions | umls-concept:C0680730 | lld:lifeskim |
| pubmed-article:11710576 | lifeskim:mentions | umls-concept:C1996904 | lld:lifeskim |
| pubmed-article:11710576 | lifeskim:mentions | umls-concept:C1880497 | lld:lifeskim |
| pubmed-article:11710576 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:11710576 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:11710576 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:11710576 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:11710576 | lifeskim:mentions | umls-concept:C1527240 | lld:lifeskim |
| pubmed-article:11710576 | lifeskim:mentions | umls-concept:C1527216 | lld:lifeskim |
| pubmed-article:11710576 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:11710576 | pubmed:dateCreated | 2001-11-16 | lld:pubmed |
| pubmed-article:11710576 | pubmed:abstractText | A selective HPLC assay is described for the determination of free and total (free plus polymer-bound) camptothecin (CPT) in human plasma after administration of the anti-tumor drug MAG-CPT (polymer bound camptothecin). Total CPT levels were determined after hydrolysis and free CPT was extracted from acidified plasma using Oasis solid-phase extraction material. Extracts were analyzed on a Zorbax SB-C8 analytical column, using a mixture of acetonitrile-25 mM phosphate buffer (pH 4.0) as the eluent. Detection was performed fluorimetrically. Concentrations of polymer-bound CPT were calculated by subtraction of free from total CPT. The lower limits of quantitation of the methods were 100 ng/ml for total and 1.0 ng/ml for free CPT using 50 microl and 250 microl plasma, respectively. Special attention was paid to the stability of the analytes. The presented method was successfully applied in a clinical pharmacokinetic study in our institute. | lld:pubmed |
| pubmed-article:11710576 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11710576 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11710576 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11710576 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11710576 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11710576 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11710576 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11710576 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:11710576 | pubmed:issn | 1387-2273 | lld:pubmed |
| pubmed-article:11710576 | pubmed:author | pubmed-author:JanseyFF | lld:pubmed |
| pubmed-article:11710576 | pubmed:author | pubmed-author:BeijnenJ HJH | lld:pubmed |
| pubmed-article:11710576 | pubmed:author | pubmed-author:RosingHH | lld:pubmed |
| pubmed-article:11710576 | pubmed:author | pubmed-author:FrigerioEE | lld:pubmed |
| pubmed-article:11710576 | pubmed:author | pubmed-author:FraierDD | lld:pubmed |
| pubmed-article:11710576 | pubmed:author | pubmed-author:SchellensJ... | lld:pubmed |
| pubmed-article:11710576 | pubmed:author | pubmed-author:SchoemakerN... | lld:pubmed |
| pubmed-article:11710576 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11710576 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:11710576 | pubmed:volume | 763 | lld:pubmed |
| pubmed-article:11710576 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11710576 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11710576 | pubmed:pagination | 173-83 | lld:pubmed |
| pubmed-article:11710576 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:11710576 | pubmed:meshHeading | pubmed-meshheading:11710576... | lld:pubmed |
| pubmed-article:11710576 | pubmed:meshHeading | pubmed-meshheading:11710576... | lld:pubmed |
| pubmed-article:11710576 | pubmed:meshHeading | pubmed-meshheading:11710576... | lld:pubmed |
| pubmed-article:11710576 | pubmed:meshHeading | pubmed-meshheading:11710576... | lld:pubmed |
| pubmed-article:11710576 | pubmed:meshHeading | pubmed-meshheading:11710576... | lld:pubmed |
| pubmed-article:11710576 | pubmed:meshHeading | pubmed-meshheading:11710576... | lld:pubmed |
| pubmed-article:11710576 | pubmed:meshHeading | pubmed-meshheading:11710576... | lld:pubmed |
| pubmed-article:11710576 | pubmed:meshHeading | pubmed-meshheading:11710576... | lld:pubmed |
| pubmed-article:11710576 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11710576 | pubmed:articleTitle | High-performance liquid chromatographic analysis for the determination of a novel polymer-bound camptothecin derivative (MAG-camptothecin) and free camptothecin in human plasma. | lld:pubmed |
| pubmed-article:11710576 | pubmed:affiliation | Department of Medical Oncology, Antoni van Leeuwenhoek Hospital/The Netherlands Cancer Institute, Amsterdam. apnsc@slz.nl | lld:pubmed |
| pubmed-article:11710576 | pubmed:publicationType | Journal Article | lld:pubmed |
