| pubmed-article:11087739 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11087739 | lifeskim:mentions | umls-concept:C0036226 | lld:lifeskim |
| pubmed-article:11087739 | lifeskim:mentions | umls-concept:C0070876 | lld:lifeskim |
| pubmed-article:11087739 | lifeskim:mentions | umls-concept:C0013682 | lld:lifeskim |
| pubmed-article:11087739 | lifeskim:mentions | umls-concept:C0182537 | lld:lifeskim |
| pubmed-article:11087739 | lifeskim:mentions | umls-concept:C0547047 | lld:lifeskim |
| pubmed-article:11087739 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:11087739 | pubmed:dateCreated | 2001-5-25 | lld:pubmed |
| pubmed-article:11087739 | pubmed:abstractText | We tested the hypothesis that increased Sarcoplasmic reticulum (SR) Ca content ([Ca](SRT)) in phospholamban knockout mice (PLB-KO) is because of increased SR Ca pump efficiency defined by the steady-state SR [Ca] gradient. The time course of thapsigargin-sensitive ATP-dependent (45)Ca influx into and efflux out of cardiac SR vesicles from PLB-KO and wild-type (WT) mice was measured at 100 nm free [Ca]. We found that PLB decreased the initial SR Ca uptake rate (0.13 versus 0.31 nmol/mg/s) and decreased steady-state (45)Ca content (0.9 versus 4.1 nmol/mg protein). Furthermore, at similar total SR [Ca], the pump-mediated Ca efflux rate was higher in WT (0.065 versus 0.037 nmol/mg/s). The pump-independent leak rate constant (k(leak)) was also measured at 100 nm free [Ca]. The results indicate that k(leak) was < 1% of pump-mediated backflux and was not different among nonpentameric mutant PLB (PLB-C41F), WT pentameric PLB (same expression level), and PLB-KO. Therefore differences in passive SR Ca leak cannot be the cause of the higher thapsigargin-sensitive Ca efflux from the WT membranes. We conclude that the decreased total SR [Ca] in WT mice is caused by decreased SR Ca influx rate, an increased Ca-pump backflux, and unaltered leak. Based upon both thermodynamic and kinetic analysis, we conclude that PLB decreases the energetic efficiency of the SR Ca pump. | lld:pubmed |
| pubmed-article:11087739 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11087739 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11087739 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11087739 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11087739 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11087739 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11087739 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:11087739 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11087739 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11087739 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:11087739 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:11087739 | pubmed:author | pubmed-author:KraniasE GEG | lld:pubmed |
| pubmed-article:11087739 | pubmed:author | pubmed-author:ChiSS | lld:pubmed |
| pubmed-article:11087739 | pubmed:author | pubmed-author:BersD MDM | lld:pubmed |
| pubmed-article:11087739 | pubmed:author | pubmed-author:ShannonT RTR | lld:pubmed |
| pubmed-article:11087739 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11087739 | pubmed:day | 9 | lld:pubmed |
| pubmed-article:11087739 | pubmed:volume | 276 | lld:pubmed |
| pubmed-article:11087739 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11087739 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11087739 | pubmed:pagination | 7195-201 | lld:pubmed |
| pubmed-article:11087739 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:11087739 | pubmed:meshHeading | pubmed-meshheading:11087739... | lld:pubmed |
| pubmed-article:11087739 | pubmed:meshHeading | pubmed-meshheading:11087739... | lld:pubmed |
| pubmed-article:11087739 | pubmed:meshHeading | pubmed-meshheading:11087739... | lld:pubmed |
| pubmed-article:11087739 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11087739 | pubmed:articleTitle | Phospholamban decreases the energetic efficiency of the sarcoplasmic reticulum Ca pump. | lld:pubmed |
| pubmed-article:11087739 | pubmed:affiliation | Department of Physiology, Loyola University Chicago, Maywood, Illinois 60153, USA. | lld:pubmed |
| pubmed-article:11087739 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11087739 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:11087739 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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