Source:http://linkedlifedata.com/resource/pubmed/id/10727675
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
2000-5-8
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pubmed:abstractText |
The diagnosis and management of adults with hypercholesterolemia in the US are largely based on low-density lipoprotein cholesterol (LDL-C) concentration. In order to classify someone correctly into the National Cholesterol Education Program cut-points, LDL-C must be measured with a total error of </=12%. We examined simultaneously the analytical and clinical performance of two homogeneous LDL-C assays (LDL-C(RD), Roche Diagnostics and LDL-C(GZ), Genzyme) and the Friedewald calculation (LDL-C(Fried)). These assays correlated highly with the ultracentrifugation-dextran sulfate-Mg(2+) method (LDL-C(RD): r=0.962, y=1.029x-0.48 mmol/l, n=134; LDL-C(GZ): r=0.961, y=0.986x-0.12 mmol/l, n=134; LDL-C(Fried): r=0.960, y=1.017x-0.18 mmol/l, n=115). The total error requirement was met by the LDL-C(GZ) assay at all clinical decision cut-points, whereas the LDL-C(RD) assay met this requirement only at LDL-C concentrations of 4.92 mmol/l. The LDL-C(Fried) failed to meet the total error requirement, because the compounded imprecision of the three independent tests required for this calculation was high. Both, the LDL-C(GZ) and the LDL-C(RD) assays appeared to be only slightly affected by increasing triglycerides. At the medical decision cut-point range, the LDL-C(RD), LDL-C(GZ) and LDL-C(Fried) assays showed positive predictive values of 89-100, 85-100 and 83-99%, respectively, and negative predictive values of 52-98, 77-98 and 68-98%, respectively. The homogeneous assays provide clinical laboratories with the means to measure LDL-C in hypertriglyceridemic samples and could have a role in the diagnosis and management of hyperlipidemic patients.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol, LDL,
http://linkedlifedata.com/resource/pubmed/chemical/Chylomicrons,
http://linkedlifedata.com/resource/pubmed/chemical/Dextran Sulfate,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium,
http://linkedlifedata.com/resource/pubmed/chemical/Reagent Kits, Diagnostic,
http://linkedlifedata.com/resource/pubmed/chemical/Triglycerides
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0009-8981
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
294
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
77-92
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10727675-Adult,
pubmed-meshheading:10727675-Cholesterol,
pubmed-meshheading:10727675-Cholesterol, LDL,
pubmed-meshheading:10727675-Chylomicrons,
pubmed-meshheading:10727675-Dextran Sulfate,
pubmed-meshheading:10727675-Fasting,
pubmed-meshheading:10727675-Humans,
pubmed-meshheading:10727675-Hypercholesterolemia,
pubmed-meshheading:10727675-Hypertriglyceridemia,
pubmed-meshheading:10727675-Magnesium,
pubmed-meshheading:10727675-Predictive Value of Tests,
pubmed-meshheading:10727675-Reagent Kits, Diagnostic,
pubmed-meshheading:10727675-Regression Analysis,
pubmed-meshheading:10727675-Reproducibility of Results,
pubmed-meshheading:10727675-Triglycerides,
pubmed-meshheading:10727675-Ultracentrifugation
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pubmed:year |
2000
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pubmed:articleTitle |
Analytical performance and clinical efficacy of three routine procedures for LDL cholesterol measurement compared with the ultracentrifugation-dextran sulfate-Mg(2+) method.
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pubmed:affiliation |
Departments of Laboratory Medicine and Pathology, Children's Hospital and Harvard Medical School, Boston, MA, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study
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