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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-3-9
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pubmed:abstractText |
piwi represents the first class of genes known to be required for stem cell self-renewal in diverse organisms. In the Drosophila ovary, piwi is required in somatic signaling cells to maintain germline stem cells. Here we show that piwi encodes a novel nucleoplasmic protein present in both somatic and germline cells, with the highly conserved C-terminal region essential for its function. Removing PIWI protein from single germline stem cells significantly decreases the rate of their division. This suggests that PIWI has a second role as a cell-autonomous promoter of germline stem cell division. Consistent with its dual function, over-expression of piwi in somatic cells causes an increase both in the number of germline stem cells and the rate of their division. Thus, PIWI is a key regulator of stem cell division - its somatic expression modulates the number of germline stem cells and the rate of their division, while its germline expression also contributes to promoting stem cell division in a cell-autonomous manner.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0950-1991
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
127
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
503-14
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10631171-Animals,
pubmed-meshheading:10631171-Animals, Genetically Modified,
pubmed-meshheading:10631171-Argonaute Proteins,
pubmed-meshheading:10631171-Cell Division,
pubmed-meshheading:10631171-Cell Nucleus,
pubmed-meshheading:10631171-Drosophila,
pubmed-meshheading:10631171-Drosophila Proteins,
pubmed-meshheading:10631171-Female,
pubmed-meshheading:10631171-Genes, myc,
pubmed-meshheading:10631171-Insect Proteins,
pubmed-meshheading:10631171-Male,
pubmed-meshheading:10631171-Open Reading Frames,
pubmed-meshheading:10631171-Ovary,
pubmed-meshheading:10631171-Ovum,
pubmed-meshheading:10631171-Proteins,
pubmed-meshheading:10631171-RNA-Induced Silencing Complex,
pubmed-meshheading:10631171-Recombinant Fusion Proteins,
pubmed-meshheading:10631171-Stem Cells
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pubmed:year |
2000
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pubmed:articleTitle |
piwi encodes a nucleoplasmic factor whose activity modulates the number and division rate of germline stem cells.
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pubmed:affiliation |
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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