Linked Life Data

10618287

Source:http://linkedlifedata.com/resource/pubmed/id/10618287

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-3-2
pubmed:abstractText
Two easy-to-use commercial diagnostic assays, a dipstick enzyme-linked immunosorbent assay (ELISA) (Integrated Diagnostics, Baltimore, Md.) and an immunochromatographic card assay (PanBio, Brisbane, Australia) were evaluated for detection of immunoglobulin M (IgM) antibody to dengue virus with an in-house IgM antibody capture microplate ELISA as a reference assay. The dipstick ELISA was based on the indirect-ELISA format using dengue 2 virus as the only antigen and enzyme-labeled goat anti-human IgM antibody as the detector. The total assay time was 75 min. The immunochromatographic card assay was based on the antibody capture format and separately measured both anti-dengue virus IgM and IgG in the same test. Colloidal-gold-labeled anti-dengue virus monoclonal antibody bound with dengue virus 1 to 4 antigen cocktail was the detector, and anti-human IgM and IgG were the capture antibodies. The total assay time was <10 min. Sera from 164 individuals classified as either anti-dengue virus IgM positive (94) or anti-dengue virus IgM negative (70) in the reference microplate ELISA with a dengue virus 1 to 4 antigen cocktail were tested in the two commercial assays. The dipstick ELISA missed 7 of 94 positive samples, for a sensitivity of 92.6%, while the immunochromatographic card assay missed two positive samples, for a sensitivity of 97.9%. Of the 70 negative samples, four were false positive by the dipstick ELISA and two were false positive in the immunochromatographic card assay, resulting in specificities of 94.3 and 97.1%, respectively. Both commercial assays provide sensitive and specific detection of anti-dengue virus IgM antibody and could prove useful in settings where the microplate ELISA is impractical.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-10203534, http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-13571577, http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-2540664, http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-2667066, http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-2989309, http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-3277268, http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-6374837, http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-7625541, http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-8916809, http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-9137865, http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-9220163, http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-9431954, http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-9606000, http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-9646004, http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-9715946, http://linkedlifedata.com/resource/pubmed/commentcorrection/10618287-9742015
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1071-412X
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
106-10
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Comparison of two rapid diagnostic assays for detection of immunoglobulin M antibodies to dengue virus.
pubmed:affiliation
Viral and Rickettsial Diseases Department, Naval Medical Research Center, Bethesda, Maryland 20889-5607, USA. WuS@nmripo.nnmc.navy.mil
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, U.S. Gov't, Non-P.H.S.