Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-2-15
pubmed:abstractText
The biogenesis of giant mitochondria in flight muscle of Locusta migratoria (L.) was analyzed at the molecular level. During the 2 weeks between the beginning of the last larval stage and the imago capable of sustained flight, individual mitochondria have been shown to enlarge 30-fold and the fractional mitochondrial volume of muscle cells increases fourfold [Brosemer, R.W., Vogell, W. and Bücher, Th. (1963) Biochem. Z. 338, 854-910]. Within the same period, the activity of cytochrome c oxidase, containing subunits encoded on mitochondrial DNA, increased twofold. However, no significant change in mitochondrial DNA copy number, and even a threefold decrease in mitochondrial transcripts, was observed. Mitochondrial translation rate, measured in isolated organelles, was twofold higher in larval muscle, which can be explained only partly by the higher content of mitochondrial RNAs. Thus, rather unusually, in this system of mitochondrial differentiation, the mitochondrial biosynthetic capacity correlates with the rate of organelle biogenesis rather than the steady-state concentration of a marker enzyme. The copy number of mitochondrial DNA does not seem to play a major role in determining either mitochondrial transcript levels or functional mass.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:volume
267
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11-7
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed-meshheading:10601845-Animals, pubmed-meshheading:10601845-Artifacts, pubmed-meshheading:10601845-Blotting, Northern, pubmed-meshheading:10601845-Blotting, Southern, pubmed-meshheading:10601845-DNA, Mitochondrial, pubmed-meshheading:10601845-DNA, Superhelical, pubmed-meshheading:10601845-Electron Transport Complex IV, pubmed-meshheading:10601845-Flight, Animal, pubmed-meshheading:10601845-Gene Dosage, pubmed-meshheading:10601845-Gene Expression Regulation, pubmed-meshheading:10601845-Grasshoppers, pubmed-meshheading:10601845-Larva, pubmed-meshheading:10601845-Mitochondria, Muscle, pubmed-meshheading:10601845-Muscle, Skeletal, pubmed-meshheading:10601845-Muscle Development, pubmed-meshheading:10601845-Nucleic Acid Conformation, pubmed-meshheading:10601845-Oxidative Phosphorylation, pubmed-meshheading:10601845-Protein Biosynthesis, pubmed-meshheading:10601845-RNA, pubmed-meshheading:10601845-RNA, Messenger
pubmed:year
2000
pubmed:articleTitle
Biogenesis of giant mitochondria during insect flight muscle development in the locust, Locusta migratoria (L.). Transcription, translation and copy number of mitochondrial DNA.
pubmed:affiliation
Deaprtment of Physiology II, University of Heidelberg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't