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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1999-9-8
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pubmed:databankReference |
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pubmed:abstractText |
A self-cloning system for Actinomadura verrucosospora, a producer of the angucyclic antibiotic pradimicin A (PRM A), has been developed. The system is based on reproducible and reliable protoplasting and regeneration conditions for A. verrucosospora and a novel plasmid vector that consists of a replicon from a newly found Actinomadura plasmid and a selectable marker cloned from the Actinomadura strain. The system has an efficiency of more than 10(5) CFU/microgram of DNA. Using this system, we have cloned and identified the polyketide synthase (PKS) genes essential for PRM A biosynthesis from A. verrucosospora. Nucleotide sequence analysis of the 3.5-kb SalI-SphI fragment showed that ketosynthase subunits (open reading frame 1 [ORF1] and ORF2) of the essential PKS genes have strong similarities (59 to 89%) to those for angucyclic antibiotic biosynthesis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-1494349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-1554361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-1557033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-1938902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-2231712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-2515104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-3162770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-3198502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-4585795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-6096212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-7592377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-7881555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-8478257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-8478259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-8534971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-9224566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10347064-9339544
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2703-9
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:10347064-Actinomycetales,
pubmed-meshheading:10347064-Amino Acid Sequence,
pubmed-meshheading:10347064-Anthracyclines,
pubmed-meshheading:10347064-Antibiotics, Antineoplastic,
pubmed-meshheading:10347064-Antifungal Agents,
pubmed-meshheading:10347064-Cloning, Molecular,
pubmed-meshheading:10347064-DNA, Bacterial,
pubmed-meshheading:10347064-Molecular Sequence Data,
pubmed-meshheading:10347064-Multienzyme Complexes,
pubmed-meshheading:10347064-Nucleic Acid Hybridization,
pubmed-meshheading:10347064-Plasmids,
pubmed-meshheading:10347064-Protoplasts,
pubmed-meshheading:10347064-Sequence Alignment,
pubmed-meshheading:10347064-Sequence Analysis, DNA,
pubmed-meshheading:10347064-Transformation, Bacterial
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pubmed:year |
1999
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pubmed:articleTitle |
Development of a self-cloning system for Actinomadura verrucosospora and identification of polyketide synthase genes essential for production of the angucyclic antibiotic pradimicin.
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pubmed:affiliation |
Biotechnology Research Center, Toyama Prefectural University, Kurokawa 5180, Kosugi, Toyama 939-0398, Japan. dairi@pu-toyama.ac.jp
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pubmed:publicationType |
Journal Article
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