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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1999-5-19
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pubmed:abstractText |
SigB, a newly discovered alternative sigma factor of Staphylococcus aureus, has been shown to play an important role in stress responses and the regulation of virulence factors. The rsbW (orf159) gene is immediately upstream of sigB. Its gene product is homologous to Bacillus subtilis RsbW which under appropriate conditions binds to B. subtilis SigB and functions as an anti-sigma factor or negative posttranslational regulator. To define the function of S. aureus RsbW, both the S. aureus SigB and RsbW proteins were expressed in Escherichia coli and purified. Cross-linking experiments with these purified proteins revealed that RsbW was capable of specific binding to SigB. In an in vitro transcription runoff assay, RsbW prevented SigB-directed transcription from the sar P3 promoter, a known SigB-dependent promoter, and the inhibitory activity of RsbW was found to be concentration dependent. We also identified SigB promoter consensus sequences upstream of the genes encoding alkaline shock protein 23 and coagulase and have demonstrated SigB and RsbW dependence for the promoters in vitro. These results show that RsbW is a protein sequestering anti-sigma factor of S. aureus SigB and suggest that SigB activity in S. aureus is regulated posttranslationally.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-1321441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-1465428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-1655712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-1729202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-2355852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-2622397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-3481366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-4040889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-6371530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-6645989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-7507919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-7565101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-7591130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-7864904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-8144446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-8168129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-8188579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-8269394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-8358793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-8460143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-8610119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-8755885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-8824586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-8824593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-8830703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-9009298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-9251636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-9335283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-9658010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-9666957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-9683479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-9709046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-9721294,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-9733682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-9829915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-9891799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10217777-9925509
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Coagulase,
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RsbW protein, bacteria,
http://linkedlifedata.com/resource/pubmed/chemical/SigB protein, Bacteria,
http://linkedlifedata.com/resource/pubmed/chemical/Sigma Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/asp23 protein, Staphylococcus aureus
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
181
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2846-51
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:10217777-Bacterial Proteins,
pubmed-meshheading:10217777-Carrier Proteins,
pubmed-meshheading:10217777-Coagulase,
pubmed-meshheading:10217777-Cross-Linking Reagents,
pubmed-meshheading:10217777-Gene Expression Regulation, Bacterial,
pubmed-meshheading:10217777-Genes, Bacterial,
pubmed-meshheading:10217777-Promoter Regions, Genetic,
pubmed-meshheading:10217777-Protein Binding,
pubmed-meshheading:10217777-Protein Processing, Post-Translational,
pubmed-meshheading:10217777-Recombinant Proteins,
pubmed-meshheading:10217777-Sigma Factor,
pubmed-meshheading:10217777-Staphylococcus aureus,
pubmed-meshheading:10217777-Transcription, Genetic,
pubmed-meshheading:10217777-Transcription Factors
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pubmed:year |
1999
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pubmed:articleTitle |
The Staphylococcus aureus rsbW (orf159) gene encodes an anti-sigma factor of SigB.
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pubmed:affiliation |
Center for Tuberculosis Research, Department of International Health, Johns Hopkins School of Public Health, Baltimore, Maryland 21205-2179, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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