Linked Life Data

10077450

Source:http://linkedlifedata.com/resource/pubmed/id/10077450

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-5-6
pubmed:databankReference
pubmed:abstractText
Escherichia coli BM2506 produced macrolide 2'-phosphotransferase II [MPH (2') II]. A gene for MPH (2') II, designated mphB, is located on plasmids pTZ3721 and pTZ3723 in E. coli BM2506. In the present study, we determined the nucleotide sequence of the 6.5-kb EcoRI-PstI fragment containing mphB on pTZ3721. The DNA region of 6.5-kb EcoRI-PstI fragment contained five open reading frames (ORFs). ORF4 corresponded to mphB. Respective products deduced from ORF1, ORF2, ORF3, and ORF5 were similar to the penicillin-binding protein 4 of Streptomyces lactamduras, the repressor protein AcrR of the acrAB operon, the enzyme RdmC involved in the biosynthesis of the antibiotic aklavinone, and IS801 transposase-like protein from Pseudomonas pseudoalcakigenes, respectively. Among these genes, ORF2, ORF3, and mphB formed a gene cluster with ORF2 in the lead sequence. Our results suggest that mphB may originate from an operon related to antibiotic biosynthesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0918-6158
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
227-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Nucleotide sequence of the gene cluster containing the mphB gene for macrolide 2'-phosphotransferase II.
pubmed:affiliation
Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't