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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1999-2-23
|
| pubmed:abstractText |
Closure of the ductus arteriosus requires prenatal formation of intimal cushions, which occlude the vessel lumen at birth. Survival of newborns with severe congenital heart defects, however, depends on ductal patency. We used a gene transfer approach to create a patent ductus arteriosus by targeting the fibronectin-dependent smooth muscle cell migration required for intimal cushion formation. Fetal lamb ductus arteriosus was transfected in utero with hemagglutinating virus of Japan liposomes containing plasmid encoding 'decoy' RNA to sequester the fibronectin mRNA binding protein. Fibronectin translation was inhibited and intimal cushion formation was prevented. We thus established the essential role of fibronectin-dependent smooth muscle cell migration in intimal cushion formation in the intact animal and the feasibility of incorporating biological engineering in the management of congenital heart disease.
|
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
1078-8956
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
176-82
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9930865-Animals,
pubmed-meshheading:9930865-Cell Movement,
pubmed-meshheading:9930865-Disease Models, Animal,
pubmed-meshheading:9930865-Ductus Arteriosus, Patent,
pubmed-meshheading:9930865-Female,
pubmed-meshheading:9930865-Fibronectins,
pubmed-meshheading:9930865-Gene Therapy,
pubmed-meshheading:9930865-Genetic Vectors,
pubmed-meshheading:9930865-Heart Defects, Congenital,
pubmed-meshheading:9930865-Liposomes,
pubmed-meshheading:9930865-Muscle, Smooth, Vascular,
pubmed-meshheading:9930865-Plasmids,
pubmed-meshheading:9930865-Pregnancy,
pubmed-meshheading:9930865-Protein Biosynthesis,
pubmed-meshheading:9930865-RNA, Messenger,
pubmed-meshheading:9930865-RNA-Binding Proteins,
pubmed-meshheading:9930865-Respirovirus,
pubmed-meshheading:9930865-Sheep,
pubmed-meshheading:9930865-Transfection
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Gene transfer in utero biologically engineers a patent ductus arteriosus in lambs by arresting fibronectin-dependent neointimal formation.
|
| pubmed:affiliation |
Research Institute, The Hospital for Sick Children, Department of Pediatrics, University of Toronto, Ontario, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|