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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-3-31
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pubmed:abstractText |
Estrogens modulate the density of opioid receptors in selected brain areas; however, it is not clear whether they exert such an effect directly on the cells which express the opioid receptors. Therefore, we analyzed the binding of [3H]-diprenorphine in human neuroblastoma cells stably transfected with the estrogen receptor cDNA (SK-ER3 cell line). A 16-hour exposure of these cells with 1 nM 17beta-estradiol induces a progressive morphological differentiation which appears clearly established 6 days after the suspension of the treatment. The binding of [3H]-diprenorphine was then measured immediately after the exposure to 17beta-estradiol (16 h) as well as 6 days later. The results shows that the number of opioid receptors in SK-ER3 cells is unaffected at 16 h but appears significantly reduced at 6 days. This effect is blocked by the estrogen antagonist ICI-182780, and is coincident to a decrease of the inhibitory effect of morphine on cyclic AMP accumulation. Binding experiments performed using selective ligands suggest that the micro subclass of opioid receptors is down-regulated by estradiol in SK-ER3 cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Diprenorphine,
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/Morphine,
http://linkedlifedata.com/resource/pubmed/chemical/Narcotic Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Estrogen,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, delta,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, kappa,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, mu,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0028-3835
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
54-62
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9892851-Cell Differentiation,
pubmed-meshheading:9892851-Cell Division,
pubmed-meshheading:9892851-Cyclic AMP,
pubmed-meshheading:9892851-Diprenorphine,
pubmed-meshheading:9892851-Estradiol,
pubmed-meshheading:9892851-Humans,
pubmed-meshheading:9892851-Morphine,
pubmed-meshheading:9892851-Narcotic Antagonists,
pubmed-meshheading:9892851-Neuroblastoma,
pubmed-meshheading:9892851-Neurons,
pubmed-meshheading:9892851-Receptors, Estrogen,
pubmed-meshheading:9892851-Receptors, Opioid,
pubmed-meshheading:9892851-Receptors, Opioid, delta,
pubmed-meshheading:9892851-Receptors, Opioid, kappa,
pubmed-meshheading:9892851-Receptors, Opioid, mu,
pubmed-meshheading:9892851-Transfection,
pubmed-meshheading:9892851-Tritium,
pubmed-meshheading:9892851-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
Decrease of the number of opioid receptors and of the responsiveness to morphineduring neuronal differentiation induced by 17beta-estradiol in estrogen receptor-transfected neuroblastoma cells (SK-ER3).
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pubmed:affiliation |
Department of Endocrinology, Institute of Pharmacological Sciences, University of Milan, Italy. maggir@imiucca.csi.unimi.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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