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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1999-2-18
|
| pubmed:abstractText |
Experimental data indicate that mucosal CD4+ T cells play an important role in the pathogenesis of inflammatory bowel disease (IBD). Based on the pattern of cytokine production, CD4+ T cells may be distinguished into two different phenotypes. Th1 responses are characterized by secretion of interleukin (IL)-2, tumor necrosis factor (TNF)-alpha, lymphotoxin, and interferon (IFN)-gamma and are associated with delayed-type hypersensitivity reactions, whereas Th2 responses, which are characterized by secretion of IL-4, IL-5, and IL-10, have been associated with humoral immune responses and allergy. To assess the number of IFN-alpha and IL-4 positive cells in IBD and normal intestinal specimens, frozen sections from intestinal specimens from 10 Crohn's disease (CD), 8 ulcerative colitis (UC), and 8 healthy controls were examined by immunohistochemistry. Monoclonal antibodies for CD3, CD8, IFN-gamma, and IL-4 were used. T-lymphocyte infiltration and cytokine expression by epithelial, lamina propria, and submucosal cells were scored on a four-point scale by two independent observers who were blinded for the clinical data. One-way analysis of variance (ANOVA) testing was used for statistical analysis. In intestinal specimens from IBD patients, the number of CD3+ cells was found increased in the lamina propria and, within the submucosa, this increase was significant (p < 0.001). In CD the number of lamina propria IFN-gamma positive cells was significantly increased as compared with controls (p < 0.002). In UC the number of both IFN-gamma and IL-4 producing cells in the lamina propria was not significantly increased as compared with controls. The present results confirm the existence of a Th1-biased pattern production in CD but not in UC.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
1078-0998
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
285-90
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9836081-Adolescent,
pubmed-meshheading:9836081-Adult,
pubmed-meshheading:9836081-Analysis of Variance,
pubmed-meshheading:9836081-Antigens, CD,
pubmed-meshheading:9836081-Colectomy,
pubmed-meshheading:9836081-Colitis, Ulcerative,
pubmed-meshheading:9836081-Crohn Disease,
pubmed-meshheading:9836081-Culture Techniques,
pubmed-meshheading:9836081-Female,
pubmed-meshheading:9836081-Humans,
pubmed-meshheading:9836081-Immunohistochemistry,
pubmed-meshheading:9836081-Interferon-gamma,
pubmed-meshheading:9836081-Interleukin-4,
pubmed-meshheading:9836081-Intestinal Mucosa,
pubmed-meshheading:9836081-Lymphocyte Count,
pubmed-meshheading:9836081-Male,
pubmed-meshheading:9836081-Middle Aged,
pubmed-meshheading:9836081-Reference Values,
pubmed-meshheading:9836081-T-Lymphocytes
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Altered expression of interferon-gamma and interleukin-4 in inflammatory bowel disease.
|
| pubmed:affiliation |
Laboratory of Experimental Internal Medicine, Academic Medical Centre, University of Amsterdam, The Netherlands.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|