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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
1999-2-5
|
| pubmed:abstractText |
The nonclassical MHC class I HLA-G antigen is expressed in cytotrophoblasts during pregnancy and may play a role in inhibiting lysis by maternal natural killer cells. HLA-G gene transcription was analyzed in human fetal liver of 6-8 wk of gestation, a development stage where classical HLA class I expression is very reduced. We demonstrated that HLA-G transcription is undetectable in these cells and we investigated the molecular mechanisms that control the lack of HLA-G gene transcription. We compared protein interactions of nuclear extracts from first trimester fetal livers, YT2C2-PR (HLA-G negative) and JEG-3 (HLA-G positive) cell lines to a 244-bp EcoR I/Hind III DNA region located 1.2 kb from the HLA-G gene, previously shown to direct HLA-G expression in transgenic mouse placenta. A strong specific C7-factor was specifically detected in first trimester fetal liver that could account for the inhibition of HLA-G transcription. Interaction of C7-factor and cell-specific factors previously detected in YT2C2 cell line (C5, C6) with two distinct regulatory regions identify this 244-bp EcoR I/Hind III fragment as a putative target for inhibition of HLA-G transcription.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/HLA Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-G Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class I,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0198-8859
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
59
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
751-7
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9831130-Animals,
pubmed-meshheading:9831130-Cell Line,
pubmed-meshheading:9831130-DNA,
pubmed-meshheading:9831130-Female,
pubmed-meshheading:9831130-Gene Expression Regulation,
pubmed-meshheading:9831130-HLA Antigens,
pubmed-meshheading:9831130-HLA-G Antigens,
pubmed-meshheading:9831130-Histocompatibility Antigens Class I,
pubmed-meshheading:9831130-Humans,
pubmed-meshheading:9831130-Liver,
pubmed-meshheading:9831130-Mice,
pubmed-meshheading:9831130-Nuclear Proteins,
pubmed-meshheading:9831130-Pregnancy,
pubmed-meshheading:9831130-Pregnancy Trimester, First,
pubmed-meshheading:9831130-Pregnancy Trimester, Second,
pubmed-meshheading:9831130-Promoter Regions, Genetic,
pubmed-meshheading:9831130-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:9831130-Transcription, Genetic
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Specific binding of nuclear factors to the HLA-G gene promoter correlates with a lack of HLA-G transcripts in first trimester human fetal liver.
|
| pubmed:affiliation |
CEA, Service de Recherches en Hémato-immunologie, DSV, DRM, Centre Hayem, Hôpital Saint-Louis, Paris, France. moreau@dsvidf.cea.fr
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|