Linked Life Data

9828372

Source:http://linkedlifedata.com/resource/pubmed/id/9828372

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7-8
pubmed:dateCreated
1999-1-19
pubmed:abstractText
This paper reports the synthesis and screening of a combinatorial peptide library for new affinity ligands for glycosylated haemoglobin (HbA1c), which is an important indicator of diabetes control. The new ligands are suitable for large-scale synthesis and overcome the disadvantages of antibodies (unstable and expensive to produce etc.), while remaining as efficient as antibodies in binding to the analyte. The library consisted of 262,144 hexapeptides synthesised using the one-bead-one-compound technique. The hexapeptides attached onto beads were screened with glycosylated haemoglobin HbA1c. The structures of the peptides exhibiting high affinity were characterised by Edman microsequencing. Computer modelling simulation of one of the lead sequences has shown that this class of ligand has a high affinity and specificity for glycosylated haemoglobin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0956-5663
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
779-85
pubmed:dateRevised
2009-7-14
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
The synthesis and screening of a combinatorial peptide library for affinity ligands for glycosylated haemoglobin.
pubmed:affiliation
Cranfield Biotechnology Centre, Cranfield University, Bedfordshire, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't