Linked Life Data

9792519

Source:http://linkedlifedata.com/resource/pubmed/id/9792519

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-12-18
pubmed:abstractText
A postcolumn receptor-affinity detection (RAD) was developed for the detection of urokinase and cross-reactive compounds. The analytical method consisted of gradient reversed-phase HPLC coupled on-line to a RAD system based on fluorescein-labelled urokinase receptor (fluorescein-uPAR) as reagent. Fluorescein-uPAR was added continuously to the HPLC effluent to react with analytes eluting from the LC column. Unreacted fluorescein-uPAR was removed by a short affinity column packed with an immobilised urokinase support. The analyte-bound fluorescein-uPAR fraction passes the affinity column unretained and was detected downstream by means of a fluorescence detector. An absolute detection limit of 40 fmol urokinase was obtained in the flow injection mode. In the gradient HPLC-RAD system a detection limit of 40 nM (20-microl injection, absolute amount, 800 fmol) was obtained. The present method allowed the identification of active breakdown products of urokinase both in standard samples and biological matrices.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1387-2273
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
715
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
331-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Gradient reversed-phase liquid chromatography coupled on-line to receptor-affinity detection based on the urokinase receptor.
pubmed:affiliation
Leiden/Amsterdam Center for Drug Research, Division of Analytical Chemistry, University of Leiden, The Netherlands.
pubmed:publicationType
Journal Article