Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1998-11-12
pubmed:abstractText
We hypothesized that sera from type 2 diabetic patients with neuropathy contains an autoimmune immunoglobulin that promotes complement-independent, calcium-dependent apoptosis in neuronal cell lines. Neuronal cells were cultured in the presence of complement-inactivated sera obtained from patients with type 2 diabetes with and without neuropathy and healthy adult control patients. Serum from diabetic patients with neuropathy was associated with a significantly greater induction of apoptosis, compared to serum from diabetic patients without neuropathy and controls. In the presence of calcium channel antagonists, induction of apoptosis was reduced by approximately 50%. Pretreatment of neuronal cells with serum from diabetic patients with neuropathy was associated with a significant increase in elevated K+-evoked cytosolic calcium concentration. Serum-induced enhancement in cytosolic calcium and calcium current density was blocked by treatment with trypsin and filtration of the serum using a 100,000-kd molecular weight filter. Treatment with an anti-human IgG antibody was associated with intense fluorescence on the surface of neuronal cells exposed to sera from patients with type 2 diabetes mellitus with neuropathy. We conclude that sera from type 2 diabetic patients with neuropathy contains an autoimmune immunoglobulin that induces complement-independent, calcium-dependent apoptosis in neuronal cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-1374505, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-1464245, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-1694242, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-1708485, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-1853785, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-2045757, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-2164883, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-2434377, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-2592417, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-2838351, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-2989050, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-3289571, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-3838314, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-3898998, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-423756, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-599355, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-6270629, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-6811067, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-6865761, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-7473199, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-7539694, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-7589882, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-7593193, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-7645958, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-7648798, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-7686306, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-7695993, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-7781924, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-7965750, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-7981600, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-8019766, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-8078301, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-8154515, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-8306588, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-8381911, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-8515249, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-8574952, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-8636427, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-8761324, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-8858201, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-8929527, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-9039262, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-9184645, http://linkedlifedata.com/resource/pubmed/commentcorrection/9769338-9212311
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9738
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
102
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1454-62
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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