Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-11-16
pubmed:abstractText
In voltage-clamp studies of single frog skeletal muscle fibers stained with the potentiometric indicator 1-(3-sulfonatopropyl)-4-[beta[2-(di-n-octylamino)-6-naphthyl] vinyl]pyridinium betaine (di-8 ANEPPS), fluorescence transients were recorded in response to both supercharging and step command pulses. Several illumination paradigms were utilized to study global and localized regions of the transverse tubule system (T-system). The rising phases of transients obtained from global illumination regions showed distinct accelerations when supercharging pulses were applied (95% of steady-state fluorescence achieved in 1.5 ms with supercharging pulses versus 14.6 ms with step pulses). When local transients were recorded at the edge of the muscle fiber, their kinetics resembled those of the applied waveform, but a similar relationship was not observed in transients from regions near the edge chosen to minimize the surface membrane contribution. We developed a model of the T-system capable of simulating membrane potential changes as a function of time and distance along the T-system cable and the associated fluorescence changes in regions corresponding to the experimental illumination strategies. A critical parameter was the access resistance term, for which values of 110-150 Omega.cm2 were adequate to fit the data. The results suggest that the primary mechanism through which supercharging pulses boost the kinetics of T-system voltage changes most likely involves their compensating the voltage attenuation across the access resistance at the mouth of the T-tubule.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-1080153, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-10822501, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-1083424, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-13621406, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-14142170, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-14296959, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-1524823, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-2015378, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-2299329, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-2440491, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-2878439, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-310445, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-3873536, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-3968532, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-4537711, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-4544879, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-4778131, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-4812638, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-5352046, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-5352048, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-5557071, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-5699799, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-5838183, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-5840799, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-6296689, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-6334719, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-6663279, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-6802933, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-6981683, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-7811945, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-9130173, http://linkedlifedata.com/resource/pubmed/commentcorrection/9746552-9706027
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2098-116
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Supercharging accelerates T-tubule membrane potential changes in voltage clamped frog skeletal muscle fibers.
pubmed:affiliation
Department of Physiology, University of California at Los Angeles, Los Angeles, California 90095-1751, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.