Linked Life Data

9744499

Source:http://linkedlifedata.com/resource/pubmed/id/9744499

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1998-9-24
pubmed:abstractText
Quantification of metabolite or drug concentrations in living tissues requires determination of intra- and extracellular volumes. This study demonstrates how this can be achieved non-invasively by 31P magnetic resonance spectroscopy (MRS) employing dimethyl methylphosphonate (DMMP) as a marker of total water space, 3-aminopropylphosphonate (3-APP) as a marker of extracellular space and P and 3-APP as markers of intracellular pH (pH) and extracellular pH (pHe) respectively. The MRS measurements of the tumour volumes were validated by classic radiolabelling methods using 3H2O and [14C]inulin as markers of total and extracellular space respectively. The extracellular volume fraction measured by radiolabelling of RIF-1 tumours was 23 +/- 0.83% (mean +/- s.e.m. n = 9), not significantly different (P > 0.1) from that found by MRS (27 +/- 2.9%, n = 9, London, and 35 +/- 6.7, n = 14, Baltimore). In untreated RIF-1 tumours, pH was about 0.2 units higher than pHe (P < 0.01). 5-Fluorouracil (5FU) treatment (165 mg kg(-1)) caused no significant changes in either pHe or per cent extracellular volume. However significant increases in pH, 48 h after treatment (P < 0.01) correlated with decreased tumour size and improved bioenergetic status [NTP/inorganic phosphate (Pi) ratio]. This study shows the feasibility of an MR method (verified by a 'gold standard') for studying the effects of drug treatment on intra- and extracellular spaces and pH in solid tumours in vivo.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9744499-12865, http://linkedlifedata.com/resource/pubmed/commentcorrection/9744499-1333262, http://linkedlifedata.com/resource/pubmed/commentcorrection/9744499-1435214, http://linkedlifedata.com/resource/pubmed/commentcorrection/9744499-1489671, http://linkedlifedata.com/resource/pubmed/commentcorrection/9744499-1727132, http://linkedlifedata.com/resource/pubmed/commentcorrection/9744499-1798394, http://linkedlifedata.com/resource/pubmed/commentcorrection/9744499-2912937, http://linkedlifedata.com/resource/pubmed/commentcorrection/9744499-3708563, http://linkedlifedata.com/resource/pubmed/commentcorrection/9744499-6774153, http://linkedlifedata.com/resource/pubmed/commentcorrection/9744499-7142288, http://linkedlifedata.com/resource/pubmed/commentcorrection/9744499-7968440, http://linkedlifedata.com/resource/pubmed/commentcorrection/9744499-8302842, http://linkedlifedata.com/resource/pubmed/commentcorrection/9744499-8485143, http://linkedlifedata.com/resource/pubmed/commentcorrection/9744499-8554985, http://linkedlifedata.com/resource/pubmed/commentcorrection/9744499-8978630
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0007-0920
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
606-11
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Estimations of intra- and extracellular volume and pH by 31P magnetic resonance spectroscopy: effect of therapy on RIF-1 tumours.
pubmed:affiliation
Department of Radiology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't