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rdf:type |
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lifeskim:mentions |
umls-concept:C0023693,
umls-concept:C0027096,
umls-concept:C0038454,
umls-concept:C0042567,
umls-concept:C0175631,
umls-concept:C0205224,
umls-concept:C0337112,
umls-concept:C0439855,
umls-concept:C0444626,
umls-concept:C1267092,
umls-concept:C1442792,
umls-concept:C1513492,
umls-concept:C1514562,
umls-concept:C1524075,
umls-concept:C1705994,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221,
umls-concept:C2827662
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pubmed:issue |
5
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pubmed:dateCreated |
1998-10-5
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pubmed:databankReference |
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pubmed:abstractText |
The crystal structures of an expressed vertebrate smooth muscle myosin motor domain (MD) and a motor domain-essential light chain (ELC) complex (MDE), both with a transition state analog (MgADP x AIF4-) in the active site, have been determined to 2.9 A and 3.5 A resolution, respectively. The MDE structure with an ATP analog (MgADP x BeFx) was also determined to 3.6 A resolution. In all three structures, a domain of the C-terminal region, the "converter," is rotated approximately 70 degrees from that in nucleotide-free skeletal subfragment 1 (S1). We have found that the MDE-BeFx and MDE-AIF4- structures are almost identical, consistent with the fact that they both bind weakly to actin. A comparison of the lever arm positions in MDE-AIF4- and in nucleotide-free skeletal S1 shows that a potential displacement of approximately 10 nm can be achieved during the power stroke.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
559-71
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9741621-Actins,
pubmed-meshheading:9741621-Adenosine Diphosphate,
pubmed-meshheading:9741621-Amino Acid Sequence,
pubmed-meshheading:9741621-Animals,
pubmed-meshheading:9741621-Binding Sites,
pubmed-meshheading:9741621-Chickens,
pubmed-meshheading:9741621-Crystallography, X-Ray,
pubmed-meshheading:9741621-Dictyostelium,
pubmed-meshheading:9741621-Macromolecular Substances,
pubmed-meshheading:9741621-Models, Molecular,
pubmed-meshheading:9741621-Molecular Sequence Data,
pubmed-meshheading:9741621-Muscle, Smooth,
pubmed-meshheading:9741621-Myosin Light Chains,
pubmed-meshheading:9741621-Myosin Subfragments,
pubmed-meshheading:9741621-Protein Conformation,
pubmed-meshheading:9741621-Protein Structure, Tertiary
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pubmed:year |
1998
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pubmed:articleTitle |
Crystal structure of a vertebrate smooth muscle myosin motor domain and its complex with the essential light chain: visualization of the pre-power stroke state.
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pubmed:affiliation |
Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02454-9110, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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