Linked Life Data

9738060

Source:http://linkedlifedata.com/resource/pubmed/id/9738060

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1998-10-30
pubmed:abstractText
Amplification of human papillomavirus (HPV) DNA by L1 consensus primer systems (e.g., MY09/11 or GP5(+)/6(+)) can detect as few as 10 to 100 molecules of HPV targets from a genital sample. However, genotype determination by dot blot hybridization is laborious and requires at least 27 separate hybridizations for substantive HPV-type discrimination. A reverse blot method was developed which employs a biotin-labeled PCR product hybridized to an array of immobilized oligonucleotide probes. By the reverse blot strip analysis, genotype discrimination of multiple HPV types can be accomplished in a single hybridization and wash cycle. Twenty-seven HPV probe mixes, two control probe concentrations, and a single reference line were immobilized to 75- by 6-mm nylon strips. Each individual probe line contained a mixture of two bovine serum albumin-conjugated oligonucleotide probes specific to a unique HPV genotype. The genotype spectrum discriminated on this strip includes the high-risk, or cancer-associated, HPV genotypes 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 55, 56, 58, 59, 68 (ME180), MM4 (W13B), MM7 (P291), and MM9 (P238A) and the low-risk, or non-cancer-associated, genotypes 6, 11, 40, 42, 53, 54, 57, 66, and MM8 (P155). In addition, two concentrations of beta-globin probes allowed for assessment of individual specimen adequacy following amplification. We have evaluated the performance of the strip method relative to that of a previously reported dot blot format (H. M. Bauer et al., p. 132-152, in C. S. Herrington and J. O. D. McGee (ed.), Diagnostic Molecular Pathology: a Practical Approach, (1992), by testing 328 cervical swab samples collected in Digene specimen transport medium (Digene Diagnostics, Silver Spring, Md.). We show excellent agreement between the two detection formats, with 92% concordance for HPV positivity (kappa = 0.78, P < 0.001). Nearly all of the discrepant HPV-positive samples resulted from weak signals and can be attributed to sampling error from specimens with low concentrations (<1 copy/microliter) of HPV DNA. The primary advantage of the strip-based detection system is the ability to rapidly genotype HPVs present in genital samples with high sensitivity and specificity, minimizing the likelihood of misclassification.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-1657209, http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-2762325, http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-7658490, http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-7658497, http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-7665227, http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-7729939, http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-7791229, http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-7791438, http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-7839345, http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-7892889, http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-7963700, http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-8106758, http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-8220085, http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-8388478, http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-9041439, http://linkedlifedata.com/resource/pubmed/commentcorrection/9738060-9163434
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3020-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Genotyping of 27 human papillomavirus types by using L1 consensus PCR products by a single-hybridization, reverse line blot detection method.
pubmed:affiliation
Department of Human Genetics, Roche Molecular Systems, Inc., Alameda, California 94501, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.