Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1998-10-26
|
| pubmed:abstractText |
Direct evidence for the excitotoxicity of 3-N-oxalyl-L-alpha,beta-diaminopropionic acid (ODAP), the Lathyrus sativus neurotoxin has been studied by examining the binding of chemically synthesized [2,3 3H]ODAP ([3H]ODAP) to synaptic membranes. [3H]ODAP binding to membranes was mostly nonspecific, with only a very low specific binding (15-20% of the total binding) and was also not saturable. The low specific binding of [3H]ODAP remained unaltered under a variety of assay conditions. A low Bmax of 3.2 +/- 0.4 pmol/mg and Kd 0.2 +/- 0.08 microM could be discerned for the high affinity interactions under conditions wherein more than 80-90% of the binding was nonspecific. While ODAP could inhibit the binding of [3H]glutamate to chick synaptic membranes with a Ki of 10 +/- 0.9 microM, even L-DAP, a non neurotoxic amino acid was also equally effective in inhibiting the binding of [3H]glutamate. The very low specific binding of [3H]ODAP to synaptic membranes thus does not warrant considering its interactions at glutamate receptors as a significant event. The results thus suggest that the reported in vitro excitotoxic potential of ODAP may not reflect its true mechanism of neurotoxicity.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids, Diamino,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glutamate,
http://linkedlifedata.com/resource/pubmed/chemical/oxalyldiaminopropionic acid
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0364-3190
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
23
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1191-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9712190-Amino Acids, Diamino,
pubmed-meshheading:9712190-Animals,
pubmed-meshheading:9712190-Binding, Competitive,
pubmed-meshheading:9712190-Binding Sites,
pubmed-meshheading:9712190-Brain,
pubmed-meshheading:9712190-Chickens,
pubmed-meshheading:9712190-Fabaceae,
pubmed-meshheading:9712190-Glutamic Acid,
pubmed-meshheading:9712190-Kinetics,
pubmed-meshheading:9712190-Male,
pubmed-meshheading:9712190-Mice,
pubmed-meshheading:9712190-Mice, Inbred BALB C,
pubmed-meshheading:9712190-Mice, Inbred C57BL,
pubmed-meshheading:9712190-Neurotoxins,
pubmed-meshheading:9712190-Plants, Medicinal,
pubmed-meshheading:9712190-Rats,
pubmed-meshheading:9712190-Rats, Wistar,
pubmed-meshheading:9712190-Receptors, Glutamate,
pubmed-meshheading:9712190-Synaptic Membranes
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Receptor interactions of beta-N-oxalyl-L-alpha,beta-diaminopropionic acid, the Lathyrus sativus putative excitotoxin, with synaptic membranes.
|
| pubmed:affiliation |
Department of Biochemistry, O.U, Hyderabad, India.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|