Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1976-11-21
pubmed:abstractText
In a search for eucaryotic enzymes which might process the heterogenous nuclear RNA (HnRNA) from animal cells into messenger RNA, a ribonuclease called RNAse D analogous to E. coli RNAse III in its ability to cleave specifically synthetic or viral double-stranded polyribonucleotides has been detected and extensively purified from the cytosol of Krebs II mouse ascites cells. The purification procedure involved cellular fractionation followed by DEAE-and CM-cellulose chromatography and resulted in an RNAas D preparation contaminated with trace amounts of single-strand specific RNAse (equivalent to less than 0.3 ng per ml) as assayed against poly (rC). Significant levels of RNAse H activity against poly (rA)-poly (dT) were still present in these preparations.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-1089659, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-1103139, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-14284738, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-240718, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4346034, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4519651, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4531033, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4542191, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4587248, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4587249, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4592261, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4606525, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4865702, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-5367031, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-5500401, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-5700421, http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-5727127
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2055-65
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Partial purification of a double-stranded RNA specific ribonuclease (RNAse D) from Krebs II ascites cells.
pubmed:publicationType
Journal Article