rdf:type |
|
lifeskim:mentions |
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pubmed:issue |
8
|
pubmed:dateCreated |
1976-11-21
|
pubmed:abstractText |
In a search for eucaryotic enzymes which might process the heterogenous nuclear RNA (HnRNA) from animal cells into messenger RNA, a ribonuclease called RNAse D analogous to E. coli RNAse III in its ability to cleave specifically synthetic or viral double-stranded polyribonucleotides has been detected and extensively purified from the cytosol of Krebs II mouse ascites cells. The purification procedure involved cellular fractionation followed by DEAE-and CM-cellulose chromatography and resulted in an RNAas D preparation contaminated with trace amounts of single-strand specific RNAse (equivalent to less than 0.3 ng per ml) as assayed against poly (rC). Significant levels of RNAse H activity against poly (rA)-poly (dT) were still present in these preparations.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-1089659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-1103139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-14284738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-240718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4346034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4519651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4531033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4542191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4587248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4587249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4592261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4606525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-4865702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-5367031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-5500401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-5700421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/967689-5727127
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Aug
|
pubmed:issn |
0305-1048
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pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
3
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
2055-65
|
pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
|
pubmed:year |
1976
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pubmed:articleTitle |
Partial purification of a double-stranded RNA specific ribonuclease (RNAse D) from Krebs II ascites cells.
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pubmed:publicationType |
Journal Article
|