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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1998-7-9
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pubmed:abstractText |
Fyn tyrosine kinase, a member of the Src family, was recently reported to be present in neurons and glia cells. We investigated whether Fyn is involved in the Trk-dependent signal transduction pathways of neurotrophin. The Fyn-Src homology domain 2 (SH2) was observed to associate in vitro with the intracellular domain of TrkB (ICD-TrkB). This association was dependent on the autophosphorylation of ICD-TrkB. The Fyn-SH2 domains bound to phosphorylated ICD-TrkB (pICD-TrkB) with an affinity similar to the binding of phospholipase Cgamma (PLCgamma)-SH2 domains to its autophosphorylation site in TrkB. The Src-SH2 domains showed substantially lower affinity with pICD-TrkB, suggesting that the association between Fyn-SH2 and pICD-TrkB is not due to nonspecific interactions of SH2 domains with phosphorylated tyrosine residues. This is further supported by the observation that Fyn-SH2 was able to trap phosphorylated TrkB in cell lysate prepared from primary rat cortical neurons stimulated with brain-derived neurotrophic factor (BDNF). In contrast, endogenous Fyn was coprecipitated with TrkB from cortical neurons without BDNF stimulation. This basal association showed a threefold increase on BDNF stimulation, probably due to the SH2/phosphotyrosine interaction that was observed in the cell-free system. All these data suggest the involvement of Fyn in the neurotrophin signal transduction pathways downstream of TrkB.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Brain-Derived Neurotrophic Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Fyn protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Neuroprotective Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fyn,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Ciliary Neurotrophic...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nerve Growth Factor
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-3042
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
71
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
106-11
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9648856-Animals,
pubmed-meshheading:9648856-Baculoviridae,
pubmed-meshheading:9648856-Brain-Derived Neurotrophic Factor,
pubmed-meshheading:9648856-Cells, Cultured,
pubmed-meshheading:9648856-Cerebral Cortex,
pubmed-meshheading:9648856-Insects,
pubmed-meshheading:9648856-Long-Term Potentiation,
pubmed-meshheading:9648856-Neurons,
pubmed-meshheading:9648856-Neuroprotective Agents,
pubmed-meshheading:9648856-Phosphorylation,
pubmed-meshheading:9648856-Protein Binding,
pubmed-meshheading:9648856-Proto-Oncogene Proteins,
pubmed-meshheading:9648856-Proto-Oncogene Proteins c-fyn,
pubmed-meshheading:9648856-Rats,
pubmed-meshheading:9648856-Receptor, Ciliary Neurotrophic Factor,
pubmed-meshheading:9648856-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:9648856-Receptors, Nerve Growth Factor,
pubmed-meshheading:9648856-Signal Transduction,
pubmed-meshheading:9648856-src Homology Domains
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pubmed:year |
1998
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pubmed:articleTitle |
Association of the Src family tyrosine kinase Fyn with TrkB.
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pubmed:affiliation |
Novartis Pharma K.K., Research Department, Takarazuka Research Institute, Japan.
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pubmed:publicationType |
Journal Article
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