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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1998-5-6
|
| pubmed:abstractText |
Advances in the treatment of clinical disorders of mineral in homeostatis and metabolic bone disease with intact parathyroid hormone 1-84 or one of the biologically active N-terminal fragments require a precise and sensitive measurement in serum. Therefore, a two-site immunoenzymometric assay for the quantitative determination of bioactive hPTH-1-37 (human parathyroid hormone) at picomolar concentrations was developed. Monoclonal antibodies (mAB) against hPTH-1-37 were raised by hybridoma cells in serum-free cell culture. Furthermore, sequence-specific polyclonal antibodies were obtained by immunisation of rabbits using multiple antigenic peptides (MAP) representing the conspicuous regions of the primary structure of hPTH-1-37. The polyclonal and monoclonal antibodies were characterised by epitope mapping. The combination of a monoclonal antibody (13C63/5) recognising hPTH fragment 16-24 with a polyclonal antibody (k2) showing a predominant binding sequence at hPTH-1-5 led to a sandwich assay specific for N-terminally intact and therefore biologically active hPTH. The validated assay ranging from 4 to 1000 pmol/l was applied to pharmacokinetic studies of hPTH-1-37. After s.c. administration of 30 mu g/kg in 5 beagles, the maximum serum concentrations of hPTH-1-37 ranging at 2139 +/- 857 pmol/l were observed 45 min after the injection. Clearance of the peptide calculated from the exponential disappearance curve was 32.0 +/- 9.1 ml/min/kg with a mean t1/2 of 37 +/- 10 min.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0004-4172
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
48
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
199-204
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9541733-Amino Acid Sequence,
pubmed-meshheading:9541733-Animals,
pubmed-meshheading:9541733-Antibodies, Monoclonal,
pubmed-meshheading:9541733-Calibration,
pubmed-meshheading:9541733-Chinchilla,
pubmed-meshheading:9541733-Dogs,
pubmed-meshheading:9541733-Female,
pubmed-meshheading:9541733-Half-Life,
pubmed-meshheading:9541733-Humans,
pubmed-meshheading:9541733-Immunoenzyme Techniques,
pubmed-meshheading:9541733-Molecular Sequence Data,
pubmed-meshheading:9541733-Parathyroid Hormone,
pubmed-meshheading:9541733-Peptide Fragments,
pubmed-meshheading:9541733-Rabbits
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
A new immunoenzymometric assay for bioactive N-terminal human parathyroid hormone fragments and its application in pharmacokinetic studies in dogs.
|
| pubmed:affiliation |
Pharmazeutisch-Chemisches Institut Universität Heidelberg Germany.
|
| pubmed:publicationType |
Journal Article
|