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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1998-4-24
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pubmed:abstractText |
Interleukin-6 (IL-6) on target cells binds to the specific IL-6 receptor (IL-6R) and subsequently induces homodimerization of the signal-transducing protein gp130. Cells which express gp130 but no IL-6R and which therefore do not respond to IL-6 can be stimulated by the complex of IL-6 and soluble IL-6R (slL-6R). Here we show that on rat pheochromocytoma cells (PC12), the combination of IL-6 and slL-6R but not IL-6 alone induces expression of c-fos, GAP-43 and neuron-specific enolase followed by neuron-specific differentiation and formation of a neuronal network. The differentiation was dose-and time-dependent and followed the same kinetics as nerve-growth factor (NGF)-induced differentiation. The responses of PC12 cells to IL-6/sIL-6R and NGF were additive, suggesting independent signaling pathways. We demonstrate that activation of gp130 generates a neuronal differentiation signal that is equivalent to and independent of trk/NGF receptor tyrosine kinase. Interestingly, the failure of IL-6 to induce differentiation of PC12 cells is not due to lack of surface expression of IL-6R as IL-6 alone triggered expression of GAP-43 mRNA and protein. We hypothesize that PC12 cells express more gp130 than IL-6R and that the extent of activated gp130 molecules determines the quality of the response.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokine Receptor gp130,
http://linkedlifedata.com/resource/pubmed/chemical/GAP-43 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/IL6ST protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Il6st protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0953-816X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2765-73
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9517481-Animals,
pubmed-meshheading:9517481-Antibodies,
pubmed-meshheading:9517481-Antigens, CD,
pubmed-meshheading:9517481-Binding, Competitive,
pubmed-meshheading:9517481-Cell Differentiation,
pubmed-meshheading:9517481-Cytokine Receptor gp130,
pubmed-meshheading:9517481-GAP-43 Protein,
pubmed-meshheading:9517481-Gene Expression,
pubmed-meshheading:9517481-Humans,
pubmed-meshheading:9517481-Interleukin-6,
pubmed-meshheading:9517481-Membrane Glycoproteins,
pubmed-meshheading:9517481-Nerve Growth Factors,
pubmed-meshheading:9517481-Neurites,
pubmed-meshheading:9517481-Neurons,
pubmed-meshheading:9517481-Neutralization Tests,
pubmed-meshheading:9517481-PC12 Cells,
pubmed-meshheading:9517481-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:9517481-Rats,
pubmed-meshheading:9517481-Receptors, Interleukin-6,
pubmed-meshheading:9517481-Recombinant Proteins,
pubmed-meshheading:9517481-Solubility
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pubmed:year |
1997
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pubmed:articleTitle |
Activation of gp130 by IL-6/soluble IL-6 receptor induces neuronal differentiation.
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pubmed:affiliation |
I.Department of Medicine, Mainz University, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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