Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1998-1-30
|
| pubmed:abstractText |
A transplantable tumour line (SS) was established in syngeneic rats from a spontaneous fibrosarcoma that had arisen in the submandibular salivary gland of a 24-month-old male F344 rat. A cell line (SS-P) was induced from SS, and a cloned cell line (SS-A3-1) was isolated from SS-P. The primary tumour consisted of oval to spindle-shaped cells arranged in bundles with abundant collagen fibres; ultrastructurally, neoplastic cells exhibited fusiform morphology with prominent rough endoplasmic reticulum. SS tumours showed marked interlacing fascicle and herring-bone growth patterns. SS-P and SS-A3-1 were similar morphologically to each other, consisting of oval, spindle or polygonal cells and occasional multinucleated giant cells. Tumours induced by SS-P and SS-A3-1 were histologically similar to SS tumours. Immunohistochemically, all cells in the primary tumour, SS tumours and tumours induced both by SS-P and SS-A3-1 and by SS-P and SS-A3-1 cultures gave a positive reaction to vimentin. Interestingly, neoplastic cells reacting to ED1 (rat macrophage/histiocyte-specific antibody) and alpha-smooth muscle actin (alpha-SMA) appeared in SS tumours and tumours induced by SS-P and SS-A3-1 and by SS-P and SS-A3-1 cultures. Cells with histiocytic fine structures and myofibroblastic cells with cytoplasmic actin-like microfilaments were also observed by electron microscopy. The present rat fibrosarcoma-derived transplantable tumour line (SS) and cell lines (SS-P and SS-A3-1) might express myofibroblastic and histiocytic phenotypes, probably depending on the surrounding conditions. These cell lines may prove useful for studying the mechanisms of phenotypic plasticity in neoplastic fibroblasts.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0945-6317
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
431
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
431-40
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9428931-Actins,
pubmed-meshheading:9428931-Animals,
pubmed-meshheading:9428931-Cell Division,
pubmed-meshheading:9428931-Chromosome Aberrations,
pubmed-meshheading:9428931-Female,
pubmed-meshheading:9428931-Fibroblasts,
pubmed-meshheading:9428931-Fibrosarcoma,
pubmed-meshheading:9428931-Histiocytes,
pubmed-meshheading:9428931-Histocytochemistry,
pubmed-meshheading:9428931-Immunohistochemistry,
pubmed-meshheading:9428931-Male,
pubmed-meshheading:9428931-Microscopy, Electron,
pubmed-meshheading:9428931-Muscles,
pubmed-meshheading:9428931-Neoplasm Transplantation,
pubmed-meshheading:9428931-Rats,
pubmed-meshheading:9428931-Rats, Inbred F344,
pubmed-meshheading:9428931-S100 Proteins,
pubmed-meshheading:9428931-Submandibular Gland Neoplasms,
pubmed-meshheading:9428931-Tumor Cells, Cultured,
pubmed-meshheading:9428931-Tumor Markers, Biological,
pubmed-meshheading:9428931-Vimentin
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Characteristics of a rat fibrosarcoma-derived transplantable tumour line (SS) and cultured cell lines (SS-P and SS-A3-1) showing myofibroblastic and histiocytic phenotypes.
|
| pubmed:affiliation |
Department of Veterinary Pathology, College of Agriculture, Osaka Prefecture University, Japan. yamate@jyui.vet.osakafu-u.ac.jp
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|